概述

  • 产品名称
    Anti-HLA A2抗体[BB7.2]
    参阅全部 HLA A2 一抗
  • 描述
    小鼠单克隆抗体[BB7.2] to HLA A2
  • 宿主
    Mouse
  • 特异性
    This product has been purified from tissue culture supernatant.
  • 经测试应用
    适用于: IP, Flow Cytmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
  • 免疫原

    HLA-A2 solubilised by papain

  • 表位
    The C-terminus of alpha-2 helix and a turn on one of the underlying beta strands.
  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    >95% by SDS-PAGE
  • 纯化说明
    This product has been purified from tissue culture supernatant.
  • 克隆
    单克隆
  • 克隆编号
    BB7.2
  • 同种型
    IgG2b
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab74674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
Flow Cyt Use 2µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

  • 应用说明
    Is unsuitable for WB.
  • 靶标

    • 相关性
      Involved in the presentation of foreign antigens to the immune system. Dimer of alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein.
    • 细胞定位
      Membrane; Single-pass type I membrane protein.
    • 数据库链接
    • 别名
      • Antigen presenting molecule antibody
      • HLA A antibody
      • HLA A histocompatibility type antibody
      • HLA antibody
      • HLA class I antibody
      • HLA class I histocompatibility antigen A 2 alpha chain antibody
      • HLA class I histocompatibility antigen A2 alpha chain antibody
      • HLA-A antibody
      • HLAA antibody
      • Human leukocyte antigen antibody
      • Leucocyte antigen class I antibody
      • Leukocyte antigen class I A antibody
      • Major histocompatibility complex class I A antibody
      • Major histocompatibility complex class I antibody
      • MHC class I antigen [precursor] antibody
      • MHC class I antigen A antibody
      • MHC class I antigen A*2 antibody
      • MHC class I antigen A2 antibody
      • MHC class I antigen HLA A antibody
      • MHC class I antigen HLA A heavy chain antibody
      • MHC leukocyte antigen antibody
      see all

    图片

    • Overlay histogram showing Raji cells stained with ab74674 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74674, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed Raji cells used under the same conditions.

      Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    文献

    This product has been referenced in:
    • Shafaie S  et al. Influence of surface geometry on the culture of human cell lines: A comparative study using flat, round-bottom and v-shaped 96 well plates. PLoS One 12:e0186799 (2017). Read more (PubMed: 29084285) »

    See 1 Publication for this product

    客户评价及客户问答

    Code de remise :xxxxxxx
    Date d’expiration : 08/04/12

    Je suis ravie que vous souhaitiez participer a notre offre et tester ab74674 en western blot. Le code de réduction correspondant est xxxxxxxxx. Ce code de réductio...

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    Merci de votre intérêt pour ab74674. A ma connaissance, cet anticorps n’a pas encore été testé en western blot et nous n’offrons pas d’échantillon gratuit à des fins de tests prél...

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    Thank you for contacting us.

    Unfortunately we have not determined which specific HLA-A2 allele(s) this clone binds. However, it is clear from the paper you kindly provided that the clone binds HLA-A201. Unfortunately, we currently do not have ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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