Anti-HIV1 tat抗体- ChIP Grade (ab43014)

概述

性能

应用

Our Abpromise guarantee covers the use of ab43014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 20651230
ChIP Use at an assay dependent concentration. PubMed: 22073215
CHIPseq Use at an assay dependent concentration. PubMed: 22073215
WB 1/20000. Predicted molecular weight: 14 kDa.
ELISA 1/10000.

靶标

  • 相关性HIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR.
  • 细胞定位Nuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.
  • 别名
    • p14 antibody
    • Protein Tat antibody
    • Transactivating regulatory protein antibody

Anti-HIV1 tat antibody - ChIP Grade 图像

  • All lanes : Anti-HIV1 tat antibody - ChIP Grade (ab43014) at 1/2000 dilution

    Lane 1 : Jurkat whole cell lysate 0 hours after infection
    Lane 2 : Jurkat whole cell lysate 2 hours after infection
    Lane 3 : Jurkat whole cell lysate 4 hours after infection
    Lane 4 : Jurkat whole cell lysate 6 hours after infection
    Lane 5 : Jurkat whole cell lysate 8 hours after infection
    Lane 6 : Jurkat whole cell lysate 10 hours after infection

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP Goat anti-rabbit IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 14 kDa
    Observed band size : 14 kDa

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-HIV1 tat antibody - ChIP Grade (ab43014)参考文献

This product has been referenced in:
  • Ozaki T  et al. Delivery of Topically Applied Calpain Inhibitory Peptide to the Posterior Segment of the Rat Eye. PLoS One 10:e0130986 (2015). ELISA ; Rat . Read more (PubMed: 26107400) »
  • Johnson TP  et al. Induction of IL-17 and nonclassical T-cell activation by HIV-Tat protein. Proc Natl Acad Sci U S A 110:13588-93 (2013). Read more (PubMed: 23898208) »

See all 7 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HEK293T cells)
Gel Running Conditions Reduced Denaturing (4-12)
Loading amount 10 µg
Treatment Transfected with HIV-1 NL4-3 for 48 hours
Specification HEK293T cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 19 2016

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (gel 15%)
Sample Human Cell lysate - whole cell (astrocyte U251)
Specification astrocyte U251
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 15 2013

Thank you very much for your email. My colleague is out of the office but I'll be happy to assist you with this issue.

I've looked at our stock, and unforunately we only have the same lot that did not work well for you. Did the replacement vi...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your reply and for providing the additional information.

It appears that the antibody isn't working as expected, so I would like to offer you a free of charge replacement or credit note. We only have the same lot in stock in our...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Jurkat Cells)
Loading amount 25 µg
Specification Jurkat Cells
Treatment pNL4.3 infected
Gel Running Conditions Reduced Denaturing (15% SDS-PAGE gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 01 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.



I am sorry this product did not perform as stated on the datasheet and for the inconvenie...

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DISCOUNT CODE: **********
Expiration date: **************

I am very pleased to hear you would like to accept our offer and test ab43014 in ICC/IF. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem thi...

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Thank you for your patience. I have enquired at the originator of the product but unfortunately, this product has not been tested against human macrophages and HIV strain BAL. Therefore, we do not know if it will be suitable for your research. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"