Anti-HIV1 Reverse Transcriptase抗体[AS35] (Biotin) (ab16162)

概述

  • 产品名称Anti-HIV1 Reverse Transcriptase抗体[AS35] (Biotin)
    参阅全部 HIV1 Reverse Transcriptase 一抗
  • 描述
    小鼠单克隆抗体[AS35] to HIV1 Reverse Transcriptase (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: ELISA, IPmore details
  • 免疫原

    Recombinant HIV1 Reverse Transcriptase

  • 表位Recognizes an epitope on HIV-RT that is different from AS34 epitope.

性能

应用

Our Abpromise guarantee covers the use of ab16162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
IP
  • 应用说明ELISA: This antibody has been used as solution phase ab at 2 µg/ml in a two site ELISA with AS34. Typically the ELISA has a working range of 2-500 ng/ml and a sensitivity of <1 ng/ml HIV-RT.
    IP: This antibody has been used to immunoprecipitate > 90% HIV-RT in solution using a double-antibody method. Immunoprecipitation has also been accomplished using Protein G agarose.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性HIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.
    • 别名
      • HIV 1 Reverse transcriptase antibody
      • HIV1 RT antibody
      • Human Immunodeficiency virus 1 antibody
      • p51 RT antibody
      • p66 RT antibody
      • Reverse transcriptase p51 subunit antibody
      • Reverse transcriptase p66 subunit antibody
      see all

    Anti-HIV1 Reverse Transcriptase antibody [AS35] (Biotin) (ab16162)参考文献

    This product has been referenced in:
    • Midgley AR & Hepburn MR Use of the double-antibody method to separate antibody bound from free ligand in radioimmunoassay. Methods Enzymol 70:266-74 (1980). Read more (PubMed: 7421592) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab16162.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"