Anti-HIV1 Reverse Transcriptase抗体(ab63912)

概述

  • 产品名称
    Anti-HIV1 Reverse Transcriptase抗体
    参阅全部 HIV1 Reverse Transcriptase 一抗
  • 描述
    豚鼠多克隆抗体to HIV1 Reverse Transcriptase
  • 宿主
    Guinea pig
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    Reacts with HIV1.
  • 免疫原

    HIV1 Reverse Transcriptase, over-expressed as recombinant protein in E.coli.

  • 阳性对照
    • Extract of MT4 cells infected with HIV-1 (LAI strain).

性能

应用

Our Abpromise guarantee covers the use of ab63912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2500. Detects a band of approximately 51, 66 kDa.
ELISA Use at an assay dependent concentration.

靶标

  • 相关性
    HIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.
  • 别名
    • HIV 1 Reverse transcriptase antibody
    • HIV1 RT antibody
    • Human Immunodeficiency virus 1 antibody
    • p51 RT antibody
    • p66 RT antibody
    • Reverse transcriptase p51 subunit antibody
    • Reverse transcriptase p66 subunit antibody
    see all

图片

  • All lanes : Anti-HIV1 Reverse Transcriptase antibody (ab63912) at 1/2500 dilution

    Lane 1 : Extract of MT4 cells
    Lane 2 : Extract of MT4 cells infected with HIV-1 (LAI strain)

    Observed band size: 51,66 kDa (why is the actual band size different from the predicted?)



    ab63912 detects bands of 66 kDa and 51 kDa corresponding to the two subunits of HIV1 reverse transcriptase.

实验方案

文献

ab63912 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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