The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明ELISA: 1/1000 (when antibody concentration is 1 mg/ml)
WB: 1/500 - 1/1000 (when antibody concentration is 1 mg/ml).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
相关性HIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid.
It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.