概述

  • 产品名称
    Anti-HIV1 Rev抗体[Rev-6]
    参阅全部 HIV1 Rev 一抗
  • 描述
    小鼠单克隆抗体[Rev-6] to HIV1 Rev
  • 特异性
    ab85529 detects the Rev protein of HIV1. No cross reactivity has been observed with non HIV1 Rev proteins.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    HIV
  • 免疫原

    HIV1 Rev recombinant protein (clade B, HXB-3 isolate) with an N terminal His tag, expressed in bacteria.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 0.05% Glycerol, 0.1M Sodium citrate, 0.05mM Tris, pH neutral
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 纯化说明
    Purified from supernatants of hybridoma cell cultures by affinity chromatography based on Protein A.
  • 克隆
    单克隆
  • 克隆编号
    Rev-6
  • 同种型
    IgG1
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab85529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250 - 1/500. Predicted molecular weight: 13 kDa.
ELISA 1/100 - 1/200.

靶标

  • 相关性
    HIV1 expresses the trans-activator protein Rev which is required for virus growth as it promotes the localisation and expression of viral RNA in the cytoplasm. Rev is a model experimental system for studying nuclear transport. The HIV1 Rev protein as viral protein is expressed early in the virus life cycle and thus may be important targets for the immune control of HIV1 infection.
  • 别名
    • Human immunodeficiency virus 1 Rev antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • Rev antibody

图片



  • Predicted band size : 13 kDa

    Image from Wong R et al., Retrovirology. 2011 Jun 17;8:47.Fig 8.; doi:10.1186/1742-4690-8-47; 17 June, 2011, Retrovirology, 8:47

实验方案

文献

This product has been referenced in:
  • Wong RW  et al. Digoxin suppresses HIV-1 replication by altering viral RNA processing. PLoS Pathog 9:e1003241 (2013). WB . Read more (PubMed: 23555254) »
  • Wong RW  et al. Characterization of novel inhibitors of HIV-1 replication that function via alteration of viral RNA processing and rev function. Nucleic Acids Res 41:9471-83 (2013). Human . Read more (PubMed: 23945945) »

See all 4 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 transfected with HIV-1Rev expression plasmi)
Loading amount
10 µg
Specification
HEK293 transfected with HIV-1Rev expression plasmi
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

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提交于 Jan 08 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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