Anti-HIV1 p55 + p24 + p17抗体(ab63917)

概述

  • 产品名称
    Anti-HIV1 p55 + p24 + p17抗体
  • 描述
    兔多克隆抗体to HIV1 p55 + p24 + p17
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    Reacts with HIV1.
  • 免疫原

    Recombinant protein p55 over-expressed in E.coli using a plasmid carrying the Gag p55 coding region of HIV1 virus.

  • 阳性对照
    • Extract of MT4 cells infected with HIV1 (LAI strain); HeLa whole cell lysate transfected with a plasmid expressing HIV1 gag.

性能

应用

Our Abpromise guarantee covers the use of ab63917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Detects a band of approximately 17,24,55 kDa.
ELISA Use at an assay dependent concentration.

靶标

  • 相关性
    HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. p55 is digested by HIV1 protease into intermediate products p41 and p15. p41 is further digested into matrix protein p17 and capsid protein p24. Likewise, p15 is further digested into nucleocapsid protein p7 and to p6 and p1.
  • 别名
    • CA antibody
    • Capsid protein p24 antibody
    • Gag antibody
    • Gag polyprotein antibody
    • HIV1 matrix protein p17 antibody
    • HIV1 Pr55Gag antibody
    • Human immunodeficiency virus 1 p17 antibody
    • Human immunodeficiency virus 1 p55 antibody
    • Human immunodeficiency virus type 1 p24 antibody
    • MA antibody
    • Matrix protein antibody
    • Matrix protein p17 antibody
    • Pr55 antibody
    • Pr55Gag antibody
    see all

图片

  • All lanes : Anti-HIV1 p55 + p24 + p17 antibody (ab63917) at 1/2000 dilution

    Lane 1 : Extract of MT4 cells
    Lane 2 : Extract of MT4 cells infected with HIV1
    (LAI strain)

    Observed band size: 17,24,55 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 39 kDa, 45 kDa. We are unsure as to the identity of these extra bands.

  • All lanes : Anti-HIV1 p55 + p24 + p17 antibody (ab63917) at 1/2000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate transfected with a plasmid expressing HIV1 gag
    Lane 2 : Untransfected HeLa whole cell lysate

    Secondary
    All lanes : Goat anti-rabbit IgG (whole molecule) HRP polymer at 1/50000 dilution

    See Abreview

实验方案

文献

This product has been referenced in:
  • Chai Q  et al. HIV-1 counteracts an innate restriction by amyloid precursor protein resulting in neurodegeneration. Nat Commun 8:1522 (2017). Read more (PubMed: 29142315) »
  • Malikov V & Naghavi MH Localized Phosphorylation of a Kinesin-1 Adaptor by a Capsid-Associated Kinase Regulates HIV-1 Motility and Uncoating. Cell Rep 20:2792-2799 (2017). Read more (PubMed: 28930676) »

See all 7 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
20000 cells
Specification
HeLa
Treatment
Transfected with plasmids expressing HIV gag
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

David Carpentier

Verified customer

提交于 Jan 07 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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