The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
WB: 1/2500. Detects bands of approximately 17 and 55 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA.
HIV1 Gag p17, the HIV1 matrix protein is produced by the digestion of its precursor Gag p55 by HIV1 protease.
Gag polyprotein antibody
HIV1 matrix protein p17 antibody
HIV1 Pr55Gag antibody
Human immunodeficiency virus 1 p17 antibody
Human immunodeficiency virus 1 Pr55Gag antibody
Matrix protein p17 antibody
Western blot - HIV1 p55 + HIV1 p17 antibody (ab63916)
All lanes : Anti-HIV1 p55 + p17 antibody (ab63916) at 1/2500 dilution
Lane 1 : Extract of MT4 cells Lane 2 : Extract of MT4 cells infected with HIV1 (LAI strain)