Anti-HIV1 p24抗体[ND1] (ab8246)

概述

  • 产品名称Anti-HIV1 p24抗体[ND1]
    参阅全部 HIV1 p24 一抗
  • 描述
    小鼠单克隆抗体[ND1] to HIV1 p24
  • 特异性HIV-1 p24 protein. Specificity was verified by Western Blotting in reducing conditions with Sanofi Pasteur NEW LAV-BLOT I kit.
  • 经测试应用适用于: ELISA, WBmore details
  • 免疫原

    Synthetic C-terminal peptide. (HIV)

性能

应用

Our Abpromise guarantee covers the use of ab8246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明ELISA: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
    • 细胞定位Membrane
    • 别名
      • CA antibody
      • Capsid protein p24 antibody
      • HIV-1 Gag p24 antibody
      • HIV1gp1 antibody
      • Human immunodeficiency virus 1 antibody
      • Human immunodeficiency virus type 1 p24 antibody
      see all

    Anti-HIV1 p24 antibody [ND1] (ab8246)参考文献

    This product has been referenced in:
    • Guo J  et al. Yin-Yang 1 regulates effector cytokine gene expression and T(H)2 immune responses. J Allergy Clin Immunol 122:195-201, 201.e1-5 (2008). Read more (PubMed: 18423564) »

    See 1 Publication for this product

    Product Wall

    Indeed, I can confirm that your type of experiments qualifies for the testing discount program. DISCOUNT CODES: ab73768: 100%ABR-E7TC5 ab8246: 100%ABR-E7TCE ab9071: 100%ABR-E7TCF Expiration date: 09 April 2012 I am very pleased to hear you ...

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    You are welcome! Take all the time you need. I look forward to your reply whenever you are ready.

    Thank you for your reply. Unfortunately, I would not recommend the protein ab73768. This is only a fragment of the HIV1 p24 that lacks large parts of the protein such as the N-terminal amino acids (1-76). If the antibodies you would like to test re...

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    Thank you for contacting us. The differences are as follows: ab43036 HTLV I p24 protein: This protein is the full length sequence of HTLV I p24, i.e. an in E. coli derived recombinant protein. The Human T-lymphotropic virus Type I (HTLV-1) is not...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HEK293 cells)
    Loading amount 20 µg
    Specification HEK293 cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    提交于 Nov 09 2006

    It was recombinant p24, full length

    I have enquired with the originators of these two antibodies to help answer your questions. For ab8246, the originator has provided me with following information - Ab8246 reacts with recombinant p24 (immunogen) better than with native p24. Nevertheless...

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    Thank you for your enquiry. All the information that we have regarding ab8246 is located on the online datasheet. We do not have a specific protocol for this particular antibody in ELISA. I suggest that you refer to the Abcam homepage for a general ELI...

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    It react with recombinant p24 better than with native p24. Nevertheless it does bind with native p24. We have positive results for this in Western blotting and in ELISA. For ELISA we use p24 from ABI Advanced Biotechnologies HIV-1 purified viral lysat...

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    This antibody has not yet been tested in IP.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"