概述

  • 产品名称Anti-HIV1 Nef抗体[JR6]
    参阅全部 HIV1 Nef 一抗
  • 描述
    小鼠单克隆抗体[JR6] to HIV1 Nef
  • 特异性This antibody recognizes the rNef of HIV 1 subtypes A, B and C. It reacts with HIV1 Nef of JR-CSF, BRU, IIIB, LAV & BH10 strains.
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    HIV1
  • 免疫原

    Recombinant Nef protein of HIV1 (B subtype consensus).

  • 表位Mapped to amino acids 195-205 (ARELHPEYYKD - B subtype consensus)
  • 常规说明Also known as clone 01-009.

性能

应用

Our Abpromise guarantee covers the use of ab42358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
ICC/IF
  • 应用说明ELISA: 1/25000.
    ICC/IF: Use at an assay dependent dilution.
    RipA: Use at an assay dependent dilution.
    WB: 1/5000. Predicted molecular weight: 24 kDa.

    Titer in ELISA/WB using rNEF of HIV 1 Subtype A.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation.
    • 别名
      • C terminal core protein antibody
      • F protein antibody
      • Nef antibody
      • Negative factor antibody
      • p27 antibody
      see all

    Anti-HIV1 Nef antibody [JR6] 图像

    • Lane 1 : Anti-HIV1 Nef antibody [JR6] (ab42358)
      Lane 2 :

      Lane 1 : Recominant HIV1 Nef (B subtype)
      Lane 2 : Protein size marker


      Predicted band size : 24 kDa

    Anti-HIV1 Nef antibody [JR6] (ab42358)参考文献

    This product has been referenced in:
    • Kjerrström A  et al. Interactions of single and combined human immunodeficiency virus type 1 (HIV-1) DNA vaccines. Virology 284:46-61 (2001). Read more (PubMed: 11352667) »
    • Collings A  et al. Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors. Vaccine 18:460-7 (1999). Read more (PubMed: 10519935) »

    See all 6 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"