The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 24 kDa.
Use at an assay dependent dilution.
Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation.
C terminal core protein antibody
F protein antibody
Negative factor antibody
Western blot - Anti-HIV1 Nef antibody (ab63918)
Anti-HIV1 Nef antibody (ab63918) at 1/1000 dilution + Extract of HIV-2 (strain UC2) infected cell
Predicted band size : 24 kDa
Western blot - HIV1 Nef antibody (ab63918)
All lanes : Anti-HIV1 Nef antibody (ab63918) at 1/1000 dilution
Lane 1 : Extract of MT4 cells Lane 2 : Extract of MT4 cells infected with HIV1 (LAI strain)