Anti-HIV gp160抗体(ab117122)

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概述

  • 产品名称
    Anti-HIV gp160抗体
  • 描述
    山羊多克隆抗体to HIV gp160
  • 宿主
    Goat
  • 特异性
    ab117122 reacts strongly with gp41, gp120 and gp160 in western blot assays. ELISA based peptide mapping studies have confirmed high titer reactivity to HIV MN env peptide in the following regions: (6332)IEPLGVAPTKAKRRV, (6360) CTTTVPWNASWSNKS and moderate activity to multiple other regions on the env polypeptide.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 免疫原

    Other Immunogen Type corresponding to HIV gp160. ab117122 was produced using a cocktail of three recombinant antigens: gp41, gp120, and gp41/120 hybrid.

性能

应用

Our Abpromise guarantee covers the use of ab117122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 97 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 相关性
    Endoproteolytic processing of human immunodeficiency virus type 1 (HIV1) gp160 membrane glycoprotein precursor into gp120 and gp41 is necessary for formation of infectious HIV particles. The gp120 glycoprotein is used for binding to CD4 receptor and CCR5 co-receptor of T helper 2 (Th2) cells, and is able to induce FcepsilonRI(+) hematopoietic cells to produce IL4, which inactivates the host adaptive immune response.
  • 细胞定位
    Cell Membrane
  • 别名
    • env antibody
    • Env polyprotein antibody
    • Envelope glycoprotein gp160 antibody
    • Glycoprotein 120 antibody
    • Glycoprotein 41 antibody
    • SU antibody
    • Surface protein antibody
    • Transmembrane protein gp41 antibody
    see all

实验方案

文献

ab117122 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab117122？请让我们知道，以便我们可以引用本数据表中的参考文章。

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Western blot abreview for Anti-HIV gp160 antibody

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Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T cells)
Gel Running Conditions
Reduced Denaturing (4-12)
Loading amount
10 µg
Treatment
Transfected with HIV-1 NL4-3 for 48 hours
Specification
HEK293T cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Aug 24 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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