Histone H4 Total Acetylation Detection Fast试剂盒(Colorimetric) (ab115125)

概述

  • 产品名称Histone H4 Total Acetylation Detection Fast试剂盒(Colorimetric)
    参阅全部 Histone H4 acetylation 试剂盒
  • 样品类型
    Tissue, Adherent cells, Suspension cells
  • 灵敏度
    2 ng/well
  • 范围
    5 ng/well - 2000 ng/well
  • 检测时间
    2h 30m
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: all Mammals
  • 产品概述

    Acetylation of histones such histone H4 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H4 acetylation. Reversible acetylation of nucleosomal histone H4 is believed to correlate with potential transcriptional activity of eukaryotic chromatin domains. The reversible lysine acetylation of histone H4 may play a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication and repair, nuclear import and neuronal repression.

    Histone H4 Total Acetylation Detection Fast Kit (Colorimetric) enables the user to colorimetrically detect and quantify if histone H4 is acetylated. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. Histone H4 Total Acetylation Detection Fast Kit (Colorimetric) is suitable for specifically measuring total histone H4 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

  • 经测试应用适用于: Functional Studiesmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab115125 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    实验方案

    Histone H4 Total Acetylation Detection Fast Kit (Colorimetric) (ab115125)参考文献

    This product has been referenced in:
    • Kocic G  et al. Global and specific histone acetylation pattern in patients with Balkan endemic nephropathy, a worldwide disease. Ren Fail 36:1078-82 (2014). Functional Studies ; Human . Read more (PubMed: 24845033) »

    See 1 Publication for this product

    Product Wall



    The detection antibody in ab115124 kit is labeled with signal reporter already. But H4 antibody included in the kit ab115125 is not and needs to be labeled by signal reporter and enhanced with enhancer during the assay.



    1. Yes, it is possible for tissue or cell pellets to be frozen before extracting histone proteins.

    2. The minimum amount of histone protein required as input for these assays are 50 ng (both for the colorimetric and fluorometric kits...

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    Thank you for contacting us about this exciting idea. Unfortunately our abreview program does not include kits so I cannot offer free or reduced price for this application. I encourage you to please share your findings, good or bad, with us and the...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"