Histone H4 Total Acetylation Assay 试剂盒(Colorimetric) (ab115125)
Key features and details
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 2 hr 30 min
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 2 ng/well
概述
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产品名称
Histone H4 Total Acetylation Assay 试剂盒(Colorimetric)
参阅全部 Histone H4 acetylation 试剂盒 -
检测方法
Colorimetric -
样品类型
Tissue, Adherent cells, Suspension cells -
灵敏度
2 ng/well -
范围
5 ng/well - 2000 ng/well -
检测时间
2h 30m -
种属反应性
与反应: Mouse, Human
预测可用于: Mammals -
产品概述
Acetylation of histones such histone H4 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H4 acetylation. Reversible acetylation of nucleosomal histone H4 is believed to correlate with potential transcriptional activity of eukaryotic chromatin domains. The reversible lysine acetylation of histone H4 may play a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication and repair, nuclear import and neuronal repression.
Histone H4 Total Acetylation Detection Fast Kit (Colorimetric) enables the user to colorimetrically detect and quantify if histone H4 is acetylated. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. Histone H4 Total Acetylation Detection Fast Kit (Colorimetric) is suitable for specifically measuring total histone H4 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
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平台
Microplate reader
性能
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存放说明
Please refer to protocols. -
组件 48 tests 96 tests 10X Wash Buffer 1 x 10ml 1 x 20ml 8-Well Sample Strips (with Frame) 4 units 9 units 8-Well Standard Control Strips 2 units 3 units Antibody Buffer 1 x 6ml 1 x 12ml Color Developer 1 x 5ml 1 x 10ml Detection Antibody, 1 mg/ml 1 x 5µl 1 x 10µl Signal Enhancer 1 x 120µl 1 x 240µl Signal Report Solution 1 x 5µl 1 x 10µl Standard Control (100 µg/ml) 1 x 10µl 1 x 20µl Stop Solution 1 x 3ml 1 x 6ml -
研究领域
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab115125 被引用在 4 文献中.
- Sibilia V et al. Sex Steroid Regulation of Oxidative Stress in Bone Cells: An In Vitro Study. Int J Environ Res Public Health 18:N/A (2021). PubMed: 34831936
- Casati L et al. Food for Bone: Evidence for a Role for Delta-Tocotrienol in the Physiological Control of Osteoblast Migration. Int J Mol Sci 21:N/A (2020). PubMed: 32629979
- Zhang D et al. miR-185 mediates lung epithelial cell death after oxidative stress. Am J Physiol Lung Cell Mol Physiol 310:L700-10 (2016). PubMed: 26747785
- Kocic G et al. Global and specific histone acetylation pattern in patients with Balkan endemic nephropathy, a worldwide disease. Ren Fail 36:1078-82 (2014). Functional Studies ; Human . PubMed: 24845033