Anti-Histone H4 (phospho S47)抗体(ab61061)

概述

  • 产品名称Anti-Histone H4 (phospho S47)抗体
    参阅全部 Histone H4 一抗
  • 描述
    兔多克隆抗体to Histone H4 (phospho S47)
  • 特异性ab61061 detects endogenous levels of Histone H4 only when phosphorylated at serine 47 (Human: Ser47; Mouse: Ser47).
  • 经测试应用适用于: ICC/IF, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Histone H4 aa 43-52 (phospho S47). Synthetic phosphopeptide derived from human Histone H4 around the phosphorylation site of serine 47 (RISpGL).
    Sequence:

    CVKRISGLIY


    Database link: P62805

  • 阳性对照
    • HuvEc cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab61061 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab61061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.
WB 1/1000.

靶标

Anti-Histone H4 (phospho S47) antibody 图像

  • Immunofluorescence analysis of HuvEc cells, using Histone H4 (phospho S47) antibody (ab61061) at 1/500 - 1/1000 dilution, in the presence (right panel) or absence (left panel) of phosphopeptide.
  • All lanes : Anti-Histone H4 (phospho S47) antibody (ab61061) at 1/1000 dilution

    Lane 1 : Lystaes were prepared from HeLa cells expressing rat wild type histone H4
    Lane 2 : Lystaes were prepared from Hela cells expressing rat S47A mutated histone H4

    Lysates/proteins at 5 µg per lane.

    Secondary
    HRP-conjugated goat polyclonal to rabbit IgG at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 13 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes

    This image is a courtesy of Dr K Miyake

    See Abreview

Anti-Histone H4 (phospho S47) antibody (ab61061)参考文献

ab61061 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - nuclear (Hela cells expressing rat histone H4)
Loading amount 5 µg
Specification Hela cells expressing rat histone H4
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Dr. 克英 三宅

Verified customer

提交于 Aug 07 2009

Application Western blot
Sample Human Cell lysate - whole cell (liver)
Loading amount 1 µg
Specification liver
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 17 2009

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