Histone H4 (mono-methyl K20) Quantification试剂盒(Colorimetric) (ab115093)
Key features and details
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 2 hr 30 min
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 5 ng/well
概述
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产品名称
Histone H4 (mono-methyl K20) Quantification试剂盒(Colorimetric)
参阅全部 Histone H4 试剂盒 -
检测方法
Colorimetric -
样品类型
Tissue, Adherent cells, Suspension cells -
灵敏度
5 ng/well -
范围
20 ng/well - 2000 ng/well -
检测时间
2h 30m -
种属反应性
与反应: Mouse, Human
预测可用于: Mammals -
产品概述
Methylation of histone H4 at lysine 20 is catalyzed by SET9 and SUV4.20h among other methyl transferases in mammalian cells. H4 (mono-methyl K20) is involved in the maintenance of the proper higher order structure of DNA and is consequently essential for chromosome condensation. It is also involved in gene silencing.
Histone H4 (mono-methyl K20) Quantification Kit (Colorimetric) (ab115093) enables the user to measure global mono-methylation of histone H4K20. The global H4K20 mono-methylation can also be changed by inhibition or activation of HMTs, making quantitative detection of global mono-methyl histone H4K20 useful in developing a better understanding of epigenetic regulation of gene activation/repression and for developing HMT-targeted drugs. ab115093 is suitable for use with a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
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平台
Microplate reader
性能
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存放说明
Please refer to protocols. -
组件 标识符 48 tests 96 tests 10X Wash Buffer 1 x 10ml 1 x 20ml 8-Well Sample Strips (with Frame) 4 units 9 units 8-Well Standard Control Strips Green Ringed 2 units 3 units Antibody Buffer 1 x 6ml 1 x 12ml Color Developer 1 x 5ml 1 x 10ml Detecting Antibody, 1 mg/mL 1 x 5µl 1 x 10µl Signal Enhancer 1 x 120µl 1 x 240µl Signal Report Solution 1 x 5µl 1 x 10µl Standard Control, 100 µg/mL 1 x 10µl 1 x 20µl Stop Solution 1 x 3ml 1 x 6ml -
研究领域
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功能
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
序列相似性
Belongs to the histone H4 family. -
翻译后修饰
Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression. -
细胞定位
Nucleus. Chromosome. - Information by UniProt
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别名
- dJ160A22.1
- dJ160A22.2
- dJ221C16.1
see all -
数据库链接
- Entrez Gene: 121504 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8359 Human
- Entrez Gene: 8360 Human
- Entrez Gene: 8361 Human
- Entrez Gene: 8368 Human
- Entrez Gene: 8370 Human
see all
相关产品
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Related Products
- Histone H4 (mono-methyl K20) Quantification Kit (Fluorometric) (ab115094)
- Histone H4 (di-methyl K20) Quantification Kit (Colorimetric) (ab115095)
- Histone H4 (di-methyl K20) Quantification Kit (Fluorometric) (ab115096)
- Histone H4 (tri-methyl K20) Quantification Kit (Colorimetric) (ab115097)
- Histone H4 (tri-methyl K20) Quantification Kit (Fluorometric) (ab115098)
- Histone H4 (pan-methyl K20) Quantification Kit (Colorimetric) (ab115099)
- Histone H4 (pan-methyl K20) Quantification Kit (Fluorometric) (ab115100)
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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