Anti-Histone H4 (mono methyl K20)抗体[mAbcam 16974] (ab16974)


  • 产品名称Anti-Histone H4 (mono methyl K20)抗体[mAbcam 16974]
    参阅全部 Histone H4 一抗
  • 描述
    小鼠单克隆抗体[mAbcam 16974] to Histone H4 (mono methyl K20)
  • 经测试应用适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide within Human Histone H4 aa 1-100 (mono methyl K20). The exact sequence is proprietary.
    (Peptide available as ab17043)

  • 阳性对照
    • This antibody gave a positive signal in methanol fixed/Tween permeabilised HeLa cells in Flow Cytometry.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.50
    Preservative: 0.02% Sodium azide
    Constituents: 5.88% Sodium citrate, Tris HCl, 2.9% Sodium chloride
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆单克隆
  • 克隆编号mAbcam 16974
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域


Our Abpromise guarantee covers the use of ab16974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 10 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).Can be blocked with Human Histone H4 (mono methyl K20) peptide (ab17043).
Flow Cyt Use 2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • 功能Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 序列相似性Belongs to the histone H4 family.
  • 翻译后修饰Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
    Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
    Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4.
    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
    Sumoylated, which is associated with transcriptional repression.
    Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
  • 细胞定位Nucleus. Chromosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • H4_HUMAN antibody
    • H4F2 antibody
    • H4FN antibody
    • HIST1H4 antibody
    • HIST2H4 antibody
    • Hist4h4 antibody
    • HISTH4H4 antibody
    • Histone H4 antibody
    • methyl histone H4 antibody
    • methylated histone H4 antibody
    see all

Anti-Histone H4 (mono methyl K20) antibody [mAbcam 16974] 图像

  • ELISA using ab16974 at antibody concentrations between 1/100 and 1/10000.

    The orange line indicates binding to the Histone H4 peptide - mono methyl K20 (ab17043).

    Binding to the following peptides was either not seen or was very weak:
    Histone H4 peptide - tri methyl K20 (ab17567)  - green line,
    Histone H4 peptide - di methyl K20 (ab14964)  - blue line,
    Histone H4 peptide - unmodified (ab2622) - red line.

    This indicates the specificity of ab16794 for mono methyl K20 of Histone H4.

  • Overlay histogram showing HeLa cells stained with ab16974 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16974, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Histone H4 (mono methyl K20) antibody [mAbcam 16974] (ab16974)参考文献

ab16974 has not yet been referenced specifically in any publications.