Anti-Histone H4 (di methyl K79)抗体(ab2885)

概述

  • 产品名称Anti-Histone H4 (di methyl K79)抗体
    参阅全部 Histone H4 一抗
  • 描述
    兔多克隆抗体to Histone H4 (di methyl K79)
  • 特异性Specific to Histone H4 K79 Di-methyl. In blocking experiments, is not blocked by H3 K79 peptides.
  • 经测试应用适用于: WB, IHC-P, ICC/IF, ICCmore details
  • 种属反应性
    与反应: Cow, Human
    不与反应: Saccharomyces cerevisiae
  • 免疫原

    Synthetic peptide within Human Histone H4 aa 50 to the C-terminus (di methyl K79) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    (Peptide available as ab4560)

  • 阳性对照
    • Calf Thymus Histone Preparation; Hela whole cell extract

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab2885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 14 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/200.
ICC 1/200.

靶标

  • 功能Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 序列相似性Belongs to the histone H4 family.
  • 翻译后修饰Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
    Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
    Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4.
    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
    Sumoylated, which is associated with transcriptional repression.
    Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
  • 细胞定位Nucleus. Chromosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • H4_HUMAN antibody
    • H4F2 antibody
    • H4FN antibody
    • HIST1H4 antibody
    • HIST2H4 antibody
    • Hist4h4 antibody
    • HISTH4H4 antibody
    • Histone H4 antibody
    • methyl histone H4 antibody
    • methylated histone H4 antibody
    see all

Anti-Histone H4 (di methyl K79) antibody 图像

  • All lanes : Anti-Histone H4 (di methyl K79) antibody (ab2885) at 1/500 dilution

    Lane 1 : Calf Thymus histone prep
    Lane 2 : Calf Thymus histone prep with Human Histone H3 (mono methyl K79) peptide (ab4555) at 0.1 µg
    Lane 3 : Calf Thymus histone prep with Human Histone H3 (di methyl K79) peptide (ab4556) at 0.1 µg
    Lane 4 : Calf Thymus histone prep with Human Histone H3 (tri methyl K79) peptide (ab4557) at 0.1 µg
    Lane 5 : Calf Thymus histone prep with Human Histone H4 (di methyl K79) peptide (ab4560) at 0.1 µg
    Lane 6 : Calf Thymus histone prep with Human Histone H3 (di methyl K27) peptide (ab1781) at 0.1 µg

    Lysates/proteins at 1 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

    Rabbit polyclonal to Histone H4 Di Methyl K79 at 1/500 on Calf Thymus histone prep (1 ug per lane).

    Lane 1: ab2885 1/500
    Lane 2: ab2885 1/500 H3 Mono Me K79 peptide (ab4555), 0.1 ug
    Lane 3: ab2885 1/500 H3 Di Me K79 peptide (ab4556), 0.1 ug
    Lane 4: ab2885 1/500 H3 Tri Me K79 peptide  (ab4557), 0.1 ug
    Lane 5: ab2885 1/500 H4 Di Me K79 peptide  (ab4560), 0.1 ug
    Lane 6: ab2885 1/500 H3 Di Me K27 peptide  (ab1781), 0.1 ug

    Secondary antibody: Goat anti-rabbit IgG (HRP) ab 1/2000 (ab6721)

  • SKN cells were stained with ab2885 (green) at a dilution of 1/200. The cells were fixed in paraformaldehyde for 10 minutes prior to incubation with ab2885. The DNA was stained with DAPI (blue). 100x magnification.


  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutes

    This image is courtesy of an Abreview submitted by Dr Gerald Davies

    ab2885 at 1/2000 used in Western blot analysis of MCF7 cells (human breast cancer cells).

    Lane 1: MCF7 cell lysate  control

    Lane 2: MCF7 cell lysate (cells  treated with 50µm Troglitazone for 48 hours)

    Lane 3: MCF7 cells lysate (cells treated with 1µm Trichostatin for 48 hours)

    Troglitazone and trichostatin increase dimethyl H4 (K79) and act as anti-proliferatives in human breast cancer. The blot was stripped and reprobed with an alpha tubilin antibody to show loading equivalence.

    See Abreview

  • IHC image of Histone H4 (di methyl K79) staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2885, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Histone H4 (di methyl K79) antibody (ab2885)参考文献

This product has been referenced in:
  • Zhang K  et al. Comparative analysis of histone H3 and H4 post-translational modifications of esophageal squamous cell carcinoma with different invasive capabilities. J Proteomics 112C:180-189 (2014). Read more (PubMed: 25234497) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Saccharomyces cerevisiae Purified protein (Acid extracted histones)
Loading amount 3 µg
Specification Acid extracted histones
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 12 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human MCF 7 breast cancer cells)
Loading amount 10 µg
Specification human MCF 7 breast cancer cells
Treatment 50uM TRG and 1uM Trichostatin A for 48 hours
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Gerald Davies

Verified customer

提交于 Oct 05 2006

Thank you for your query regarding ab2885. The western blot image on the datasheet was performed on purified histone preps from Calf thymus with 1 ug loading per lane (a large excess of protein). The top band relates to H3 with H2A and H2B between t...

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