Anti-Histone H4 (Biotinylated K5)抗体(ab17343)


  • 产品名称Anti-Histone H4 (Biotinylated K5)抗体
  • 描述
    兔多克隆抗体to Histone H4 (Biotinylated K5)
  • 特异性ab17343 specifically recognise biotinylated histone H4 K5 in HeLa nuclear lysates. ab17343 could not detect biotinylated histone H4 K5 in HeLa whole cell lysates.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Histone H4 aa 1-100 (N terminal) (biotinylated K5) conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab17632, ab17632)

  • 阳性对照
    • Hela nuclear extract


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域



Our Abpromise guarantee covers the use of ab17343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).


  • 功能Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 疾病相关Chromosomal aberrations involving HISTONE H4 is a cause of B-cell non-Hodgkin lymphomas (B-cell NHL). Translocation t(3;6)(q27;p21), with BCL6.
  • 序列相似性Belongs to the histone H4 family.
  • 翻译后修饰Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
    Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
    Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4.
    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
    Sumoylated, which is associated with transcriptional repression.
    Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
  • 细胞定位Nucleus. Chromosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • H4_HUMAN antibody
    • H4F2 antibody
    • H4FN antibody
    • HIST2H4 antibody
    • Hist4h4 antibody
    • Histone H4 antibody
    see all

Anti-Histone H4 (Biotinylated K5) antibody 图像

  • All lanes : Anti-Histone H4 (Biotinylated K5) antibody (ab17343) at 1 µg/ml

    Lane 1 : HeLa nuclear extract
    Lane 2 : HeLa nuclear extract with Human Histone H4 (biotinylated K5) peptide (ab17632) at 1 µg/ml
    Lane 3 : HeLa nuclear extract with Human Histone H4 peptide (ab15825) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 11 kDa
    Observed band size : 11 kDa

    See Abreview

Anti-Histone H4 (Biotinylated K5) antibody (ab17343)参考文献

ab17343 has not yet been referenced specifically in any publications.

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