概述

  • 产品名称Anti-Histone H4抗体
    参阅全部 Histone H4 一抗
  • 描述
    兔多克隆抗体to Histone H4
  • 经测试应用适用于: IP, ICC/IF, ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human Histone H4. Immunogen is in the range of aa 10-59. Synthetic non-acetylated peptide derived from human Histone H4 (Swiss-Prot P62805 ) around the acetylation site of lysine 12 (L-G-KA-G-G).
    Database link: P62805

  • 阳性对照
    • Human breast carcinoma tissue and Jurkat cell extracts treated with TSA (400nM, 24hours).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab61255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/5000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).

靶标

Anti-Histone H4 antibody 图像

  • Histone H4 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Histone H4 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab61255.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 13kDa; Histone H4

  • ab61255 at 1/50 - 1/100 dilution staining Histone H4 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-Histone H4 antibody (ab61255) at 1/500 dilution

    Lane 1 : Jurkat cell extracts treated with TSA (400nM, 24hours)
    Lane 2 : Jurkat cell extracts treated with TSA (400nM, 24hours) with the immunising peptide


    Predicted band size : 11 kDa
    Observed band size : 11 kDa
  • ICC/IF image of ab61255 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61255, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Histone H4 antibody (ab61255)参考文献

This product has been referenced in:
  • Sadeghi L  et al. The Paf1 complex factors Leo1 and Paf1 promote local histone turnover to modulate chromatin states in fission yeast. EMBO Rep 16:1673-87 (2015). WB ; Schizosaccharomyces pombe . Read more (PubMed: 26518661) »
  • Colpitts TM  et al. Dengue virus capsid protein binds core histones and inhibits nucleosome formation in human liver cells. PLoS One 6:e24365 (2011). WB, ICC/IF ; Human . Read more (PubMed: 21909430) »

See all 2 Publications for this product

Product Wall

Yes, the antibody can also recongize the acetylated histone H4. This antibody has been tested this antibody by ELISA and can react with Acetyl peptide and Non-acetyl peptide. The results are here below: Dilution 1:1000 - 1:4...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"