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Synthetic peptide corresponding to Human Histone H4. Immunogen is in the range of aa 10-59.
Synthetic non-acetylated peptide derived from human Histone H4 (Swiss-Prot P62805 ) around the acetylation site of lysine 12 (L-G-KA-G-G).
Database link: P62805
Our Abpromise guarantee covers the use of ab61255 in the following tested applications.
|IP||Use a concentration of 5 µg/ml.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).|
Histone H4 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Histone H4 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab61255.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 13kDa; Histone H4