概述

  • 产品名称Anti-Histone H4抗体
    参阅全部 Histone H4 一抗
  • 描述
    兔多克隆抗体to Histone H4
  • 经测试应用适用于: ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human Histone H4. Immunogen is in the range of aa 6-55. Synthetic non-acetylated peptide derived from human Histone H4 (Swiss-Prot P62805) around the acetylation site of lysine 8 (G-G-KA-G-L).
    Database link: P62805

  • 阳性对照
    • Human breast carcinoma tissue. ICC/IF: HeLa cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab61254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 1 µg/ml.

靶标

Anti-Histone H4 antibody 图像

  • ab61254 staining Histone H4 in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab61254 at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 2 μg/ml (shown in green). AlexaFluor®350 WGA was used at a 1/200 dilution and incubated for 1h with the cells, to label plasma membranes (shown in blue). Nuclear DNA was labelled in red with 1.25 μM DRAQ5™ (ab108410), which was added to the secondary antibody mixture. A secondary only negative control is displayed, which indicates that the Histone H4 staining observed is due to primary antibody specificity and not to unspecific binding of the secondary antibody to the cells.

  • ab61254 at 1/50 - 1/100 dilution staining Histone H4 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

Anti-Histone H4 antibody (ab61254)参考文献

ab61254 has not yet been referenced specifically in any publications.

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