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Recombinant full length protein corresponding to Saccharomyces cerevisiae Histone H4 aa 1 to the C-terminus.
Database link: P02309
Our Abpromise guarantee covers the use of ab16483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa).|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 20709786ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
ab16483 detects a band of approximately 14 kDa in Calf thymus histone prep, HeLa histone prep and recombinant Histone H4. This is slightly larger than the predicted molecular weight according to Swissprot (11 kDa), however we are confident that ab16483 is recognising Histone H4.