RabMAb

Anti-Histone H4 (acetyl K5)抗体[EP1000Y] - ChIP Grade (ab51997)

概述

  • 产品名称Anti-Histone H4 (acetyl K5)抗体[EP1000Y] - ChIP Grade
    参阅全部 Histone H4 一抗
  • 描述
    兔单克隆抗体[EP1000Y] to Histone H4 (acetyl K5) - ChIP Grade
  • 经测试应用适用于: ChIP, WB, IHC-P, ICC/IF, IPmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human, Xenopus laevis, Rice
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Histone H4 aa 1-100 (N terminal) (acetyl K5).
    Database link: P62805

  • 阳性对照
    • HeLa cells or human brain glioma. IHC-P: Human normal colon FFPE tissue sections.
  • 常��说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Alternative versions available :

    Anti-Histone H4 (acetyl K5) antibody (Biotin) [EP1000Y] (ab202886)

性能

应用

Our Abpromise guarantee covers the use of ab51997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration. PubMed: 18354499
WB 1/10000 - 1/50000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
IP 1/50.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    Anti-Histone H4 (acetyl K5) antibody [EP1000Y] - ChIP Grade 图像

    • IHC image of ab51997 staining Histone H4 (acetyl K5) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51997, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • All lanes : Anti-Histone H4 (acetyl K5) antibody [EP1000Y] - ChIP Grade (ab51997) at 1/1000000 dilution

      Lane 1 : Untreated HeLa cells
      Lane 2 : TSA treated HeLa calls

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP labelled (1:2000)

      Predicted band size : 11 kDa
      Observed band size : 11 kDa
    • ICC/IF image of ab51997 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51997, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Anti-Histone H4 (acetyl K5) antibody [EP1000Y] - ChIP Grade (ab51997)参考文献

    This product has been referenced in:
    • Kim DW  et al. A histone deacetylase inhibitor improves hypothyroidism caused by a TRa1 mutant. Hum Mol Genet 23:2651-64 (2014). Mouse . Read more (PubMed: 24381310) »
    • Ren Y  et al. Potential of adipose-derived mesenchymal stem cells and skeletal muscle-derived satellite cells for somatic cell nuclear transfer mediated transgenesis in Arbas Cashmere goats. PLoS One 9:e93583 (2014). IHC ; Goat . Read more (PubMed: 24699686) »

    See all 18 Publications for this product

    Product Wall

    Application Flow Cytometry
    Sample Mouse Cell (Thymus)
    Permeabilization Yes - FIX & PERM® Cell Fixation & Cell Permeabilization Kit
    Gating Strategy Thymocytes (CD45+ Epcam- cells)
    Specification Thymus
    Fixation FIX & PERM® Cell Fixation & Cell Permeabilization Kit
    Username

    Abcam user community

    Verified customer

    提交于 May 20 2015

    We listed ChIP on the datasheet based on a reference that used the antibody for this application.

    Knutson SK et al. Liver-specific deletion of histone deacetylase 3 disrupts metabolic transcriptional networks. EMBO J 27:1017-28 (2008).
    Read More

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Xenopus laevis Cell (Fibroblast)
    Specification Fibroblast
    Fixative Paraformaldehyde
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 05 2013

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Schizosaccharomyces pombe Purified protein (Purified modified histone peptides)
    Loading amount 0.1 µg
    Specification Purified modified histone peptides
    Gel Running Conditions Non-reduced Non-Denaturing (Native)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 Feb 03 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"