Recombinant fragment corresponding to Human Histone H3.3. Recombinant protein fragment corresponding to a region within amino acids 1 and 106 of Human Histone H3.3B (AAH01124).
Database link: P84243
Our Abpromise guarantee covers the use of ab97968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
WB | 1/500 - 1/3000. Predicted molecular weight: 15 kDa. | |
ICC/IF | Use a concentration of 5 µg/ml. | |
IHC-P | 1/100 - 1/500. |
ICC/IF image of ab97968 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab97968 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"