Anti-Histone H3 (tri methyl K9, phospho S10)抗体- ChIP Grade (ab5819)

概述

  • 产品名称Anti-Histone H3 (tri methyl K9, phospho S10)抗体- ChIP Grade
    参阅全部 Histone H3 一抗
  • 描述
    兔多克隆抗体to Histone H3 (tri methyl K9, phospho S10) - ChIP Grade
  • 经测试应用适用于: IHC-P, ChIP, WB, ICC/IF, ICCmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human Histone H3 aa 1-100 (methyl K9, phospho S10) conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab15644)

  • 阳性对照
    • HeLa Histone Preparation; Colcemid-treated HeLa histone Preparation; Hela whole cell extract

性能

应用

Our Abpromise guarantee covers the use of ab5819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ChIP Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 17 kDa).Can be blocked with Human Histone H3 (tri methyl K9 or phospho S10) peptide (ab15644).
ICC/IF Use at an assay dependent concentration.
ICC 1/2000.

靶标

Anti-Histone H3 (tri methyl K9, phospho S10) antibody - ChIP Grade 图像

  • SKN cells stained with ab5819 (green) at a dilution of 1/2000. The cells were fixed in paraformaldehyde for 10 minutes prior to incubation with ab5819. The DNA is stained with DAPI (blue). 100x magnification.

    SKN cells stained with ab5819 (green) at a dilution of 1/2000. The cells were fixed in paraformaldehyde for 10 minutes prior to incubation with ab5819. The DNA is stained with DAPI (blue). 100x magnification.

  • ab5819 at a 1/500 dilution staining mitotic human HeLa cells by immunocytochemistry. The antibody was incubated with formaldehyde fixed cells for 1 1/2 hours and bound antibody was then detected using a Texas red conjugated goat anti-rabbit IgG.

    This image is courtesy of an Anonymous Abreview submitted on 6 April 2006.

    See Abreview

  • IHC image of Histone H3 (tri methyl K9, phospho S10) staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5819, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Histone H3 (tri methyl K9, phospho S10) antibody - ChIP Grade (ab5819)参考文献

This product has been referenced in:
  • Sabbattini P  et al. An H3K9/S10 methyl-phospho switch modulates Polycomb and Pol II binding at repressed genes during differentiation. Mol Biol Cell 25:904-15 (2014). ChIP ; Mouse . Read more (PubMed: 24430871) »
  • Malik P  et al. NET23/STING promotes chromatin compaction from the nuclear envelope. PLoS One 9:e111851 (2014). Read more (PubMed: 25386906) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
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提交于 Apr 06 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"