Histone H3 (tri-methyl K4) Quantification试剂盒(Fluorometric) (ab115057)

概述

  • 产品名称Histone H3 (tri-methyl K4) Quantification试剂盒(Fluorometric)
    参阅全部 Histone H3 (K4 methylation) 试剂盒
  • 样品类型
    Tissue, Adherent cells, Suspension cells
  • 检测类型Quantitative
  • 检测时间
    2h 30m
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: all Mammals
  • 产品概述

    Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions. Enrichment of H3 (tri-methyl K4) is found at transcription start sites of active genes where they contribute to transcriptional initiation.

     Histone H3 (tri-methyl K4) Quantification Kit (Fluorometric) (ab115057) allows the user to specifically measure global tri-methylation of histone H3K4 fluorometrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

  • 经测试应用适用于: Functional Studiesmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab115057 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    Histone H3 (tri-methyl K4) Quantification Kit (Fluorometric) 图像

    • Histone extracts were prepared from MDA-231 cells using the Histone Extraction Kit (ab113476) and the amount of trimethyl-H3-K4 was measured using ab115057.

    实验方案

    Histone H3 (tri-methyl K4) Quantification Kit (Fluorometric) (ab115057)参考文献

    ab115057 has not yet been referenced specifically in any publications.

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