Histone H3 (tri methyl K27) Assay试剂盒(In Situ) (ab115045)

概述

  • 产品名称Histone H3 (tri methyl K27) Assay试剂盒(In Situ)
    参阅全部 Histone H3 (K27 methylation) 试剂盒
  • 样品类型
    Adherent cells
  • 检测类型Cell-based (quantitative)
  • 检测时间
    3h 00m
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: all Mammals
  • 产品概述

    Methylation of histone H3 at lysine 27 has an important role in transcription repression and it is catalyzed by G9a and Polycomb Groups enzymes such as EZH2. In particular, tri-methylation of histone H3 at lysine 27 (H3 (tri methyl K27)) is a facultative heterochromatin mark, which promotes the recruitment of polycomb group proteins for gene silencing. Increased global histone H3 (tri methyl K27) is also found to be involved in some pathological processes such as cancer progression.

    Histone H3 (tri methyl K27) Assay Kit (In Situ) (ab115045) allows the user to specifically measure histone H3K27me3 in situ using cultured adherent cells in a quick and efficient procedure which can be finished within 3 hours.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 存放说明Please refer to protocols.
  • 组件 96 tests 2 x 96 tests
    10X Wash Buffer 1 x 30ml 2 x 30ml
    30% H2O2 Solution 1 x 0.5ml 1 x 1ml
    8-Well Control Strips 2 units 4 units
    Antibody Buffer 1 x 10ml 1 x 20ml
    Blocking Buffer 1 x 20ml 2 x 20ml
    Capture Antibody, 1000 µg/mL 1 x 9µl 1 x 18µl
    Detection Antibody, 200 µg/mL 1 x 10µl 1 x 20µl
    Developing Solution 1 x 12ml 1 x 24ml
    H3K27me3 Control, 20 µg/mL 1 x 15µl 1 x 30µl
    Microplate 1 unit 2 units
    Permeabilizing Buffer 1 x 30ml 2 x 30ml
    Stop Solution 1 x 6ml 1 x 12ml
  • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab115045 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    Histone H3 (tri methyl K27) Assay Kit (In Situ) 图像

    • ab115045 detecting Histone H3 (tri methyl K27) in MDA-231 cancer cells. Cells were grown in microplate wells for 48 hours.

    实验方案

    Histone H3 (tri methyl K27) Assay Kit (In Situ) (ab115045)参考文献

    ab115045 has not yet been referenced specifically in any publications.

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