Histone H3 Total Quantification试剂盒(Colorimetric) (ab115091)

概述

  • 产品名称Histone H3 Total Quantification试剂盒(Colorimetric)
    参阅全部 Histone H3 试剂盒
  • 样品类型
    Tissue, Adherent cells, Suspension cells
  • 灵敏度
    10 ng/well
  • 范围
    20 ng/well - 1000 ng/well
  • 检测时间
    2h 30m
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: all Mammals
  • 产品概述

    Histone H3 can undergo several epigenetic modifications that influence cellular processes such as transcription activation/inactivation, chromosome packaging, and DNA damage/repair. These modifications including acetylation, phosphorylation or methylation occur on the N-terminal tail domains of histone H3 through a variety of histone modifying enzymes. In most species, histone H3 is primarily acetylated at K9, K14, K18, K23 and K56, methylated at K4, K9, K27, K36 and K79, and phosphorylated at S10, S28, T3 and T11, respectively.

    Abcam's Histone H3 Total Quantification Kit (Colorimetric) (ab115091) enables the user to quantify levels of histone H3 proteins independent of their modified states. It can also be used for normalizing the modified histone H3 content of samples when run in parallel with Abcam's histone modification quantification kit series. ab115091 is suitable for specifically measuring total histone H3 on mammalian samples such as human, mouse, and rat, including fresh and frozen tissues and cultured adherent and suspension cells.

  • 经测试应用适用于: Functional Studiesmore details

性能

应用

Our Abpromise guarantee covers the use of ab115091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

实验方案

Histone H3 Total Quantification Kit (Colorimetric) (ab115091)参考文献

ab115091 has not yet been referenced specifically in any publications.

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