Histone H3 (phospho S28) Assay试剂盒(Fluorometric) (ab115130)

概述

  • 产品名称Histone H3 (phospho S28) Assay试剂盒(Fluorometric)
    参阅全部 Histone H3 (S28 Phosphorylation) 试剂盒
  • 样品类型
    Tissue, Adherent cells, Suspension cells
  • 检测类型Quantitative
  • 灵敏度
    0.5 ng/well
  • 检测时间
    3h 00m
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: all Mammals
  • 产品概述

    Phosphorylation of histone H3 at serine 28 is conserved through eukaryotes, and an increase in phosphorylation has been shown to correlate with gene activation and cell growth. In vitro studies have shown that phosphorylation of histone H3 at both S10 and S28 is coupled with dynamic acetylation of H3, where histone H3 (phospho S28) had a higher steady state of acetylation than that of H3 (phospho S10). H3 (phospho S28) is regulated by the cell cycle and has been used as a mitotic marker.

    Abcam's Histone H3 (phospho S28) Assay Kit (Fluorometric) (ab115130) allows the user to measure global histone H3 (phospho S28) quickly and consistently. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment and it suitable for specifically measuring global histone H3 phophorylation at Ser28 using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

  • 经测试应用适用于: Functional Studiesmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab115130 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    实验方案

    Histone H3 (phospho S28) Assay Kit (Fluorometric) (ab115130)参考文献

    ab115130 has not yet been referenced specifically in any publications.

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