Histone H3 (phospho S10) Assay试剂盒(In Situ) (ab115126)

概述

  • 产品名称
    Histone H3 (phospho S10) Assay试剂盒(In Situ)
    参阅全部 Histone H3 (S10 Phosphorylation) 试剂盒
  • 样品类型
    Adherent cells
  • 检测类型
    Cell-based (quantitative)
  • 检测时间
    3h 00m
  • 种属反应性

    预测可用于: Mammal
  • 产品概述

    Phosphorylation of histone H3 at serine 10 is conserved through eukaryotes, and an increase in phosphorylation has been shown to correlate with gene activation and cell growth. Histone H3 (phospho S10) is regulated by the cell cycle and has been used as a mitotic marker. Detection in the change of histone H3 (phospho S10) associated with the cell cycle, apoptosis, inhibitor or activator treatment would provide useful information for better understanding the pathological process of some diseases.

    Abcam's Histone H3 (phospho S10) Assay Kit (In Situ) (ab115126) allows the user to measure histone H3 (phospho S10) in situ using cultured adherent cells in a quick and efficient procedure which can be finished within 3 hours.

  • 经测试应用
    适用于: Functional Studiesmore details

性能

应用

Our Abpromise guarantee covers the use of ab115126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

图片

  • Log-phase MCF-7 cells were serum-starved overnight, and serum was added back for 30 minutes followed by treatment with Calyculin A (CL-A, 0.2 µM for 30 minutes). The level of H3 phosphorylation at ser10 was measured with ab115126.

实验方案

文献

ab115126 has not yet been referenced specifically in any publications.

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