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Synthesized phosphopeptide derived from human Histone H3 around the phosphorylation site of serine 10 (R-K-SP-T-G)
Our Abpromise guarantee covers the use of ab47297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/500. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||1/2000. See Abreview.|
|WB||1/500 - 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).|
|IHC-P||Use at an assay dependent concentration.|
ab47297 staining human breast carcinoma by IHC-P (left hand panel). The right hand panel shows staining in the presence of immunizing phosphopeptide.
ab47297 staining Histone H3 (phospho S10) in embryonic mouse brain by IHC-Fr (Paraformaldehyde-fixed Frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% TX-100 and blocked with 10% serum for 1 hour at 25°C. Samples were incubated with primary antibody 1/2000 (10%NGS in PBS + 0.1% TX10) for 16 hours at 25°C. A goat anti-rabbit Alexa Fluor® 488 (ab150077), dilution 1/400, was used as secondary antibody. The image shows ab47297 staining (green) in the CNS ventricular zone; there was no background staining with ab47297.
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