Histone H3 (pan-methyl K9) Quantification试剂盒(Fluorometric) (ab115067)

概述

  • 产品名称Histone H3 (pan-methyl K9) Quantification试剂盒(Fluorometric)
    参阅全部 Histone H3 (K9 methylation) 试剂盒
  • 测试
    1 x 96 test
  • 样品类型
    Tissue, Adherent cells, Suspension cells
  • 检测类型Quantitative
  • 灵敏度
    0.4 ng/well
  • 范围
    5 ng/well - 2000 ng/well
  • 检测时间
    3h 00m
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: all Mammals
  • 产品概述

    Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases is involved in euchromatin gene silencing as well as heterochromatin formation. H3 (mono-methyl K9) is enriched in certain euchromatic domains, which have been postulated to be transcriptionally silent. H3 (di-methyl K9) and H3 (tri-methyl K9) mediate heterochromatin formation by forming a binding site for HP1 and also participate in silencing gene expression at euchromatic sites.

    Histone H3 (pan-methyl K9) Quantification Kit (Fluorometric) (ab115067) allows the user to specifically measure global mono-, di-, and tri-methylation of histone H3K9 fluorometrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

  • 经测试应用适用于: Functional Studiesmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab115067 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    Histone H3 (pan-methyl K9) Quantification Kit (Fluorometric) 图像

    • ab115067 used to specifically measure global histone H3K9 mono-, di-, and tri-methylation. The ratio of mono-, di-, and tri-methylated H3K9 is proportional to the intensity of fluorescence. The absolute amount of the methylated H3K9 can be quantitated by comparing to the standard control.

    实验方案

    Histone H3 (pan-methyl K9) Quantification Kit (Fluorometric) (ab115067)参考文献

    ab115067 has not yet been referenced specifically in any publications.

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