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Methylation of histone H3 at lysine 79 is catalyzed by Dot1 in mammalian cells. Intergenic H3 (mono-methyl K79) is a mark of enhancer regions and may provide unique binding surfaces along the chromatin fibre to stabilize interactions between enhancers and their target promoters. H3 (di-methyl K79) and H3 (tri-methyl K79) are also widespread histone modifications and are associated with transcriptionally active genes. Increased global H3 (methyl K79) is also found to be involved in some pathological processes such as leukemogenesis in humans.
Histone H3 (pan-methyl K79) Quantification Kit (Fluorometric) (ab115090) allows the user to specifically measure global mono-, di-, and tri-methylation of histone H3K79 fluorometrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
|10X Wash Buffer||1 x 20ml|
|8-Well Sample Strips (with Frame)||9 units|
|8-Well Standard Control Strips||Green Ringed||3 units|
|Antibody Buffer||1 x 12ml|
|Detection Antibody, 1 mg/mL||1 x 10µl|
|Fluoro Developer||1 x 24µl|
|Fluoro Dilution||1 x 8ml|
|Fluoro Enhancer||1 x 24µl|
|Standard Control, 100 µg/mL||1 x 20µl|
Our Abpromise guarantee covers the use of ab115090 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115090 has not yet been referenced specifically in any publications.