Histone H3 (mono-methyl K9) Quantification试剂盒(Fluorometric) (ab115061)

概述

  • 产品名称Histone H3 (mono-methyl K9) Quantification试剂盒(Fluorometric)
    参阅全部 Histone H3 (K9 methylation) 试剂盒
  • 样品类型
    Tissue, Adherent cells, Suspension cells
  • 检测类型Quantitative
  • 检测时间
    2h 30m
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: all Mammals
  • 产品概述

    Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases mediates heterochromatin formation by forming a binding site for HP1. H3 (mono-methyl K9) is enriched in certain euchromatin domains which have been postulated to be transcriptionally silent.

    Histone H3 (mono-methyl K9) Quantification Kit (Fluorometric) (ab115061) allows the user to specifically measure global mono-methylation of histone H3K9 fluorometrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

  • 经测试应用适用于: Functional Studiesmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab115061 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    Histone H3 (mono-methyl K9) Quantification Kit (Fluorometric) 图像

    • Histone extracts were prepared from MDA-231 cells using the Histone Extraction Kit (ab113476) and the amount of mono-methyl H3K9 was measured using ab115061.

    实验方案

    Histone H3 (mono-methyl K9) Quantification Kit (Fluorometric) (ab115061)参考文献

    ab115061 has not yet been referenced specifically in any publications.

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