Histone H3 (mono-methyl K27) Quantification试剂盒(Colorimetric) (ab115068)

概述

  • 产品名称Histone H3 (mono-methyl K27) Quantification试剂盒(Colorimetric)
    参阅全部 Histone H3 (K27 methylation) 试剂盒
  • 样品类型
    Tissue, Adherent cells, Suspension cells
  • 检测类型Quantitative
  • 灵敏度
    2 ng/well
  • 范围
    20 ng/well - 5000 ng/well
  • 检测时间
    2h 30m
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: all Mammals
  • 产品概述

    Methylation of histone H3 at lysine 27 (K27) is a mark of gene inactivation and it is thought to mediate heterochromatin formation by forming a binding site for Polycomb. H3 (methyl K27) is also thought to have an important role in the repression of HOX genes during development and in X chromosome inactivation and imprinting. H3 (mono-methyl K27), a modification enriched at pericentromeric heterochromatin, was observed to be broadly distributed throughout all euchromatic sites and participates in silencing gene expression.
    Histone H3 (mono-methyl K27) Quantification Kit (Colorimetric) (ab115068) allows the user to specifically measure global mono-methylation of histone H3-K27 colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

  • 经测试应用适用于: Functional Studiesmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab115068 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    Histone H3 (mono-methyl K27) Quantification Kit (Colorimetric) 图像

    • ab115068 used to measure the amount of mono-methyl H3K27. Histone extracts were prepared from MDA-MB-231 cells using the Histone Extraction Kit (ab113476).

    实验方案

    Histone H3 (mono-methyl K27) Quantification Kit (Colorimetric) (ab115068)参考文献

    ab115068 has not yet been referenced specifically in any publications.

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