Histone H3 (methyl K9) Assay试剂盒(In Situ) (ab115047)

概述

  • 产品名称Histone H3 (methyl K9) Assay试剂盒(In Situ)
    参阅全部 Histone H3 (K9 methylation) 试剂盒
  • 样品类型
    Adherent cells
  • 检测类型Cell-based (quantitative)
  • 检测时间
    3h 00m
  • 种属反应性
    与反应: Mouse
    预测可用于: all Mammals
  • 产品概述

    Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites. Moreover, presence of H3 (methyl K9) positively correlates with DNA methylation.

    Histone H3 (methyl K9) Assay Kit (In Situ) (ab115047) allows the user to specifically measure histone H3K9me in situ using cultured adherent cells in a quick and efficient procedure which can be finished within 3 hours.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 存放说明Please refer to protocols.
  • 组件 96 tests 2 x 96 tests
    10X Wash Buffer 1 x 30ml 2 x 30ml
    30% H2O2 Solution 1 x 0.5ml 1 x 1ml
    8-Well Control Strips 2 units 4 units
    Antibody Buffer 1 x 15ml 1 x 20ml
    Blocking Buffer 1 x 20ml 2 x 20ml
    Capture Antibody, 100 µg/mL 1 x 60µl 1 x 120µl
    Detection Antibody, 400 µg/mL 10 x 20µl 1 x 40µl
    Developing Solution 1 x 12ml 1 x 24ml
    Methylated H3K9 Control, 20 µg/mL 1 x 15µl 1 x 30µl
    Microplates 1 unit 2 units
    Permeabilizing Buffer 1 x 30ml 2 x 30ml
    Stop Solution 1 x 6ml 1 x 12ml
  • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab115047 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    实验方案

    Histone H3 (methyl K9) Assay Kit (In Situ) (ab115047)参考文献

    ab115047 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab115047.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"