Histone H3 (methyl K4) Assay试剂盒(In Situ) (ab115046)

概述

  • 产品名称Histone H3 (methyl K4) Assay试剂盒(In Situ)
    参阅全部 Histone H3 (K4 methylation) 试剂盒
  • 样品类型
    Adherent cells
  • 检测类型Cell-based (quantitative)
  • 检测时间
    3h 00m
  • 产品概述

    Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions and may serve as a global epigenetic mark in euchromatin. H3 (methyl K4) also appears to have an inverse correlation to allele-specific DNA methylation.

    Histone H3 (methyl K4) Assay Kit (In Situ) (ab115046) allows the user to specifically measure histone H3K4 methylation in situ using cultured adherent cells in a quick and efficient procedure which can be finished within 3 hours.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 存放说明Please refer to protocols.
  • 组件 96 tests 2 x 96 tests
    10X Wash Buffer 1 x 30ml 2 x 30ml
    30% H2O2 Solution 1 x 0.5ml 1 x 1ml
    8-Well Control Strips 2 units 4 units
    Antibody Buffer 1 x 10ml 1 x 20ml
    Blocking Buffer 1 x 20ml 2 x 20ml
    Capture Antibody, 100 µg/mL 1 x 60µl 1 x 120µl
    Detection Antibody, 200 µg/mL 1 x 20µl 1 x 40µl
    Developing Solution 1 x 12ml 1 x 24ml
    Methylated H3K4 Control, 20 µg/mL 1 x 15µl 1 x 30µl
    Microplates 1 unit 2 units
    Permeabilizing Buffer 1 x 30ml 2 x 30ml
    Stop Solution 1 x 6ml 1 x 12ml
  • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab115046 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    实验方案

    Histone H3 (methyl K4) Assay Kit (In Situ) (ab115046)参考文献

    ab115046 has not yet been referenced specifically in any publications.

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