Histone H3 (K9) Methyltransferase Activity Quantification Assay试剂盒(ab113453)
Key features and details
- Assay type: Quantitative
- Platform: Microplate reader
- Assay time: 3 hr
- Sample type: Cell Lysate, Tissue Extracts
概述
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产品名称
Histone H3 (K9) Methyltransferase Activity Quantification Assay试剂盒
参阅全部 Histone Methyltransferase 试剂盒 -
样品类型
Tissue Extracts, Cell Lysate -
检测类型
Quantitative -
检测时间
3h 00m -
种属反应性
与反应: Mouse, Human
预测可用于: Mammals -
产品概述
Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites. In mammalian cells, histone lysine methyltransferases such as SuVar39h and ESET among others are responsible for methylation of lysine 9 at histone H3.
Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (ab113453) allows the user to measure the activity or inhibition of individual histone methyltransferase (HMT) that specifically target histone H3 at lysine 9 (H3K9). The kit is ready-to-use and provides all the essential components needed to carry out a successful HMT activity/inhibition quantification experiment without the need for radioactivity or any special equipment.
ab113453 recognizes methylated H3 at lysine site 9.
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平台
Microplate reader
性能
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存放说明
Please refer to protocols. -
组件 48 tests 96 tests 10X Wash Buffer 1 x 11ml 1 x 22ml 8-Well Assay Strips (with Frame) 6 units 12 units Adomet 1 x 25µl 1 x 50µl Biotinylated Substrate, 25 µg/mL 1 x 100µl 1 x 200µl Capture Antibody, 100 µg/mL 1 x 25µl 1 x 50µl Control Enzyme (300 µg/mL) 1 x 5µl 1 x 10µl Detection Antibody, 200 µg/mL 1 x 10µl 1 x 20µl Developing Solution 1 x 6ml 1 x 12ml Histone Assay Buffer 1 x 1.5ml 1 x 3ml HMT Standard, 10 µg/mL 1 x 10µl 1 x 20µl Stop Solution 1 x 3ml 1 x 6ml -
研究领域
相关产品
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Related Products
图片
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Nuclear extracts were prepared from MCF-7 cells using the ab113474 and H3-K9 specific histone methyltrasferase activity (G9a) was measured. Purified G9a was spiked into the nuclear extract that has low concentration of nuclear proteins by dilution.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab113453 被引用在 2 文献中.
- Srinivasan SP et al. Epigenetic mechanisms of Strip2 in differentiation of pluripotent stem cells. Cell Death Discov 8:447 (2022). PubMed: 36335090
- Ogawa S et al. SETDB1 Inhibits p53-Mediated Apoptosis and Is Required for Formation of Pancreatic Ductal Adenocarcinomas in Mice. Gastroenterology 159:682-696.e13 (2020). PubMed: 32360551