Histone H3 (K4) Methyltransferase Activity Quantification Assay试剂盒(ab113452)

概述

  • 产品名称Histone H3 (K4) Methyltransferase Activity Quantification Assay试剂盒
    参阅全部 Histone Methyltransferase 试剂盒
  • 检测类型Quantitative
  • 检测时间
    3h 00m
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: all Mammals
  • 产品概述

    Methylation of histone H3 at lysine 4 (K4) is thought to be a global epigenetic mark in euchromatin and a mediator of activated transcription. In mammalian cells, several SET-domain containing histone lysine methyltransferases such as MLL1 are responsible for adding one, two or three methyl groups at lysine 4 of histone H3.
    Histone H3 (K4) Methyltransferase Activity Quantification Assay Kit (ab113452) allows the user to measure the activity or inhibition of individual histone methyltransferases that specifically target histone H3 at lysine 4 (H3K4). The kit is ready-to-use and provides all the essential components needed to carry out a successful histone methyltransferase activity/inhibition quantification assay without the need for radioactivity or any special equipment.

  • 经测试应用适用于: Functional Studiesmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab113452 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    Histone H3 (K4) Methyltransferase Activity Quantification Assay Kit 图像

    • Nuclear extracts were prepared from MCF-7 cells using the ab113474 and H3-K4 specific histone methyltrasferase activity (SET7/9) was measured.

    实验方案

    Histone H3 (K4) Methyltransferase Activity Quantification Assay Kit (ab113452)参考文献

    ab113452 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you contacting us with these further questions. I have spoken with the lab about a usefull concentration for the purified HMT enzyme. Based on tests, we conclude that the useful concentration range can be from 10 ng to 200 ng/well (30 ul). ...

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    Thank you for contacting us. I have had a chance to speak with the lab regarding your question. They have told me that the purified HMT enzyme (H3-K4 specific) can be used in this assay kit. I hope this information is helpful to you. Please do ...

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    Thank you for your enquiry regarding ab113452.
    1. The biotinylated substrate is unmethylated histone peptide and used for H3-K9 specific histone methyltransferase reaction.
    2. For generating a standard curve, first, dilute HK5 HMT Standard wi...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"