Anti-Histone H3 (acetyl K9, phospho S10)抗体- ChIP Grade (ab12181)

概述

  • 产品名称Anti-Histone H3 (acetyl K9, phospho S10)抗体- ChIP Grade
    参阅全部 Histone H3 一抗
  • 描述
    兔多克隆抗体to Histone H3 (acetyl K9, phospho S10) - ChIP Grade
  • 特异性Staining of (acetyl K9) & (phospho S10) histone H3 in immunoblotting is specifically inhibited with the immunizing peptide. There is no inhibition with non-acetylated histone H3 peptide or with singly modified (acetyl K9) or (phospho S10) histone H3 peptide.
  • 经测试应用适用于: ICC/IF, ChIP, WBmore details
  • 种属反应性
    与反应: Mouse, Chicken, Human, Drosophila melanogaster
    预测可用于: Rat, Cow, Xenopus laevis, Caenorhabditis elegans, a wide range of other species
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).

  • 阳性对照
    • interphase HepG2 cells (see Abreview)
  • 常规说明Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Whole antiserum is fractionated and then further purified by ion-exchange chromatography. The resulting IgG fraction is further purified by absorption on the unmodified histone H3 peptide (human, amino acids 7-20).
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab12181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000.
ChIP Use at an assay dependent concentration. PubMed: 17675409
WB 1/1000. Detects a band of approximately 17 kDa.

靶标

  • 相关性Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination, are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by the modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways. Phosphorylation, particularly that of histones H1 and H3, has long been implicated in chromosome condensation during mitosis. However, converging evidence suggests that H3 phosphorylation (specifically serine 10) is also directly correlated with the induction of immediate-early genes such as c-jun, c-fos and c-myc. The potential importance of the serine 10 phosphorylation mark in H3 is strengthened by the finding that MSK1, a kinase activated by growth factor and stress stimuli, also phosphorylates H3 in vitro. H3 phosphorylation at serine 10 in conjunction with phosphorylation at serine 28, is also required for proper segregation and condensation of chromosomes during mitosis and meiosis.
  • 数据库链接
    see all
  • 别名
    • H3 3 like sequence MH921 antibody
    • H3 3A antibody
    • H3 histone antibody
    • H3 histone family member E pseudogene antibody
    • H3F3 antibody
    • H3F3A antibody
    • HIST3H3 antibody
    • Histone H3 3 pseudogene antibody
    see all

Anti-Histone H3 (acetyl K9, phospho S10) antibody - ChIP Grade 图像

  • This image was kindly supplied as part of the review submitted by Monika Harrer.
  • All lanes : Anti-Histone H3 (acetyl K9, phospho S10) antibody - ChIP Grade (ab12181)

    Lane 1 : Whole cell extract of NIH3T3 cells
    Lane 2 : Whole cell extract of NIH3T3 cells with [Ac-Lys9, pSer10] Histone H3 peptide
    Lane 3 : Whole cell extract of NIH3T3 cells with [non-Ac, non-phos] Histone H3 peptide
    Lane 4 : Whole cell extract of NIH3T3 cells with [Ac-Lys9] Histone H3 peptide
    Lane 5 : Whole cell extract of NIH3T3 cells with [pSer10] Histone H3 peptide

    Secondary
    Alkaline phosphatase conjugated Goat anti-Rabbit developed with a colorimetric substrate

    Observed band size : 17 kDa (why is the actual band size different from the predicted?)

Anti-Histone H3 (acetyl K9, phospho S10) antibody - ChIP Grade (ab12181)参考文献

This product has been referenced in:
  • Carter SD  et al. Distinct epigenetic and gene expression changes in rat hippocampal neurons after Morris water maze training. Front Behav Neurosci 9:156 (2015). ChIP ; Rat . Read more (PubMed: 26136669) »
  • Mostocotto C  et al. Poly(ADP-ribosyl)ation is required to modulate chromatin changes at c-MYC promoter during emergence from quiescence. PLoS One 9:e102575 (2014). ChIP ; Human . Read more (PubMed: 25047032) »

See all 9 Publications for this product

Product Wall

Application IHC - Wholemount
Sample Fruit fly (Drosophila melanogaster) Embryo (Fruit fly embryo)
Specification Fruit fly embryo
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Verified customer

提交于 Nov 13 2013

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12)
Sample Human Cell lysate - whole cell (Human Hela cells)
Specification Human Hela cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Oct 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - nuclear (HeLa)
Specification HeLa
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step Real-time PCR
Positive control GAPDH promoter
Negative control bglobin promoter
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Abcam user community

Verified customer

提交于 Jul 22 2010

I have double checked with my colleagues and we believe that the antibody will not recognise singly modified H3, as there was no inhibition with non-acetylated histone H3 peptide or with singly modified (acetyl K9) or (phospho S10) histone H3 peptide.

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