Histone H3 (acetyl K9) Assay试剂盒(In Situ) (ab115101)

概述

  • 产品名称Histone H3 (acetyl K9) Assay试剂盒(In Situ)
    参阅全部 Histone H3 (K9 acetylation) 试剂盒
  • 样品类型
    Adherent cells
  • 检测类型Cell-based (quantitative)
  • 检测时间
    3h 00m
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: all Mammals
  • 产品概述

    Acetylation of histone H3 at lysine 9 (K9) is a mark of active chromatin and appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Increased H3 (acetyl K9) may also reflect a decrease in H3 (methyl K9) associated with gene repression.

    Histone H3 (acetyl K9) Assay Kit (In Situ) (ab115101) allows the user to measure in situ acetylation of histone H3K9 at tremendously fast speeds and consistency, superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. ab115101 is suitable for specifically measuring in situ histone H3K9 acetylation using cultured adherent cells.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 存放说明Please refer to protocols.
  • 组件 96 tests 2 x 96 tests
    10X Wash Buffer 1 x 30ml 2 x 30ml
    30% H2O2 Solution 1 x 0.5ml 1 x 1ml
    8-Well Control Strips 2 units 4 units
    Antibody Buffer 1 x 15ml 1 x 30ml
    Blocking Buffer 1 x 20ml 2 x 20ml
    Capture Antibody, 1 mg/mL 1 x 6µl 1 x 10µl
    Detection Antibody, 400 µg/mL 1 x 20µl 1 x 40µl
    Developing Solution 1 x 12ml 1 x 24ml
    H3K9ac Control, 20 µg/mL 1 x 15µl 1 x 30µl
    Microplates 1 unit 2 units
    Permeabilizing Buffer 1 x 30ml 2 x 30ml
    Stop Solution 1 x 6ml 1 x 12ml
  • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab115101 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    实验方案

    Histone H3 (acetyl K9) Assay Kit (In Situ) (ab115101)参考文献

    ab115101 has not yet been referenced specifically in any publications.

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