RabMAb

Anti-Histone H3 (acetyl K9)抗体[Y28] (ab32129)

概述

  • 产品名称Anti-Histone H3 (acetyl K9)抗体[Y28]
    参阅全部 Histone H3 一抗
  • 描述
    兔单克隆抗体[Y28] to Histone H3 (acetyl K9)
  • 特异性This antibody may be cross reactive with other acetyl sites such as k4, k14 and k18.
  • 经测试应用适用于: WB, IHC-P, ICC, IPmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Histone H3 aa 1-100.
    Database link: P68431

  • 阳性对照
    • NIH 3T3 cell lysate and human lung carcinoma IHC-P: Human normal colon FFPE tissue sections.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-Histone H3 (acetyl K9) antibody [Y28] (Alexa Fluor® 647) (ab200989)

性能

应用

Our Abpromise guarantee covers the use of ab32129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/250.
IP 1/40.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    • 序列相似性Belongs to the histone H3 family.
    • 发展阶段Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
    • 翻译后修饰Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
      Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
      Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
      Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
      Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
      Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
    • 细胞定位Nucleus. Chromosome.
    • Information by UniProt
    • 数据库链接
      see all
    • 别名
      • H3.3A antibody
      • HIST1 cluster, H3E antibody
      • H3 histone family, member A antibody
      • H3.1 antibody
      • H3/l antibody
      • H3F3 antibody
      • H3FF antibody
      • H3FJ antibody
      • H3FL antibody
      • Histone gene cluster 1, H3 histone family, member E antibody
      • histone H3.1t antibody
      • Histone H3/o antibody
      • FLJ92264 antibody
      • H 3 antibody
      • H3 antibody
      • H3 histone family, member B antibody
      • H3 histone family, member C antibody
      • H3 histone family, member D antibody
      • H3 histone family, member F antibody
      • H3 histone family, member H antibody
      • H3 histone family, member I antibody
      • H3 histone family, member J antibody
      • H3 histone family, member K antibody
      • H3 histone family, member L antibody
      • H3 histone family, member T antibody
      • H3 histone, family 3A antibody
      • H3/A antibody
      • H3/b antibody
      • H3/c antibody
      • H3/d antibody
      • h3/f antibody
      • H3/h antibody
      • H3/i antibody
      • H3/j antibody
      • H3/k antibody
      • H3/t antibody
      • H31_HUMAN antibody
      • H3F1K antibody
      • H3F3A antibody
      • H3FA antibody
      • H3FB antibody
      • H3FC antibody
      • H3FD antibody
      • H3FH antibody
      • H3FI antibody
      • H3FK antibody
      • HIST1 cluster, H3A antibody
      • HIST1 cluster, H3B antibody
      • HIST1 cluster, H3C antibody
      • HIST1 cluster, H3D antibody
      • HIST1 cluster, H3F antibody
      • HIST1 cluster, H3G antibody
      • HIST1 cluster, H3H antibody
      • HIST1 cluster, H3I antibody
      • HIST1 cluster, H3J antibody
      • HIST1H3A antibody
      • HIST1H3B antibody
      • HIST1H3C antibody
      • HIST1H3D antibody
      • HIST1H3E antibody
      • HIST1H3F antibody
      • HIST1H3G antibody
      • HIST1H3H antibody
      • HIST1H3I antibody
      • HIST1H3J antibody
      • HIST3H3 antibody
      • Histone 1, H3a antibody
      • Histone 1, H3b antibody
      • Histone 1, H3c antibody
      • Histone 1, H3d antibody
      • Histone 1, H3e antibody
      • Histone 1, H3f antibody
      • Histone 1, H3g antibody
      • Histone 1, H3h antibody
      • Histone 1, H3i antibody
      • Histone 3, H3 antibody
      • histone cluster 1 H3 family member a antibody
      • histone cluster 1 H3 family member b antibody
      • histone cluster 1 H3 family member c antibody
      • histone cluster 1 H3 family member d antibody
      • histone cluster 1 H3 family member e antibody
      • histone cluster 1 H3 family member f antibody
      • histone cluster 1 H3 family member g antibody
      • histone cluster 1 H3 family member h antibody
      • histone cluster 1 H3 family member i antibody
      • histone cluster 1 H3 family member j antibody
      • Histone cluster 1, H3a antibody
      • Histone cluster 1, H3b antibody
      • Histone cluster 1, H3c antibody
      • Histone cluster 1, H3d antibody
      • Histone cluster 1, H3e antibody
      • Histone cluster 1, H3f antibody
      • Histone cluster 1, H3g antibody
      • Histone cluster 1, H3i antibody
      • Histone cluster 1, H3j antibody
      • Histone gene cluster 1, H3 histone family, member A antibody
      • Histone gene cluster 1, H3 histone family, member B antibody
      • Histone gene cluster 1, H3 histone family, member C antibody
      • Histone gene cluster 1, H3 histone family, member D antibody
      • Histone gene cluster 1, H3 histone family, member F antibody
      • Histone gene cluster 1, H3 histone family, member G antibody
      • Histone gene cluster 1, H3 histone family, member H antibody
      • Histone gene cluster 1, H3 histone family, member I antibody
      • Histone gene cluster 1, H3 histone family, member J antibody
      • Histone gene cluster 1, H3A antibody
      • Histone gene cluster 1, H3B antibody
      • Histone gene cluster 1, H3C antibody
      • Histone gene cluster 1, H3D antibody
      • Histone gene cluster 1, H3E antibody
      • Histone gene cluster 1, H3F antibody
      • Histone gene cluster 1, H3G antibody
      • Histone gene cluster 1, H3H antibody
      • Histone gene cluster 1, H3I antibody
      • Histone gene cluster 1, H3J antibody
      • Histone H 3 antibody
      • Histone H3.1 antibody
      • Histone H3.2 antibody
      • Histone H3.3 antibody
      • Histone H3/a antibody
      • Histone H3/b antibody
      • Histone H3/c antibody
      • Histone H3/d antibody
      • Histone H3/f antibody
      • Histone H3/h antibody
      • Histone H3/i antibody
      • Histone H3/j antibody
      • Histone H3/k antibody
      • Histone H3/l antibody
      • Histone H3/m antibody
      see all

    Anti-Histone H3 (acetyl K9) antibody [Y28] 图像

    • IHC image of ab32129 staining Histone H3 (acetyl K9) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32129, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • All lanes : Anti-Histone H3 (acetyl K9) antibody [Y28] (ab32129) at 1/500 dilution

      Lane 1 : Untreated NIH 3T3 cell lysate
      Lane 2 : NIH 3T3 cell lysate treated with TSA


      Predicted band size : 15 kDa
    • Immunohistochemical analysis of paraffin-embedded human lung carcinoma using ab32129 at 1/50 - 1/100

    Anti-Histone H3 (acetyl K9) antibody [Y28] (ab32129)参考文献

    This product has been referenced in:
    • Juengel E  et al. HDAC-inhibition counteracts everolimus resistance in renal cell carcinoma in vitro by diminishing cdk2 and cyclin A. Mol Cancer 13:152 (2014). WB . Read more (PubMed: 24935000) »
    • Gil R  et al. SIRT6 exhibits nucleosome-dependent deacetylase activity. Nucleic Acids Res 41:8537-8545 (2013). WB ; Human . Read more (PubMed: 23892288) »

    See all 3 Publications for this product

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