RabMAb

Anti-Histone H3 (acetyl K27)抗体[EP865Y] (ab45173)

概述

  • 产品名称Anti-Histone H3 (acetyl K27)抗体[EP865Y]
    参阅全部 Histone H3 一抗
  • 描述
    兔单克隆抗体[EP865Y] to Histone H3 (acetyl K27)
  • 特异性There is cross-reactivity with H2BK5Ac (Histone H2B acetylated on Lys 5).
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human, Saccharomyces cerevisiae
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Histone H3 aa 1-100 (acetyl K27).

  • 阳性对照
    • A431 cells and human hepatocellular carcinoma.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

性能

应用

Our Abpromise guarantee covers the use of ab45173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 18 kDa (predicted molecular weight: 15 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    • 序列相似性Belongs to the histone H3 family.
    • 发展阶段Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
    • 翻译后修饰Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
      Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
      Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
      Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
      Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
      Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
    • 细胞定位Nucleus. Chromosome.
    • Information by UniProt
    • 数据库链接
      see all
    • 别名
      • H3.3A antibody
      • HIST1 cluster, H3E antibody
      • H3 histone family, member A antibody
      • H3.1 antibody
      • H3/l antibody
      • H3F3 antibody
      • H3FF antibody
      • H3FJ antibody
      • H3FL antibody
      • Histone gene cluster 1, H3 histone family, member E antibody
      • histone H3.1t antibody
      • Histone H3/o antibody
      • FLJ92264 antibody
      • H 3 antibody
      • H3 antibody
      • H3 histone family, member B antibody
      • H3 histone family, member C antibody
      • H3 histone family, member D antibody
      • H3 histone family, member F antibody
      • H3 histone family, member H antibody
      • H3 histone family, member I antibody
      • H3 histone family, member J antibody
      • H3 histone family, member K antibody
      • H3 histone family, member L antibody
      • H3 histone family, member T antibody
      • H3 histone, family 3A antibody
      • H3/A antibody
      • H3/b antibody
      • H3/c antibody
      • H3/d antibody
      • h3/f antibody
      • H3/h antibody
      • H3/i antibody
      • H3/j antibody
      • H3/k antibody
      • H3/t antibody
      • H31_HUMAN antibody
      • H3F1K antibody
      • H3F3A antibody
      • H3FA antibody
      • H3FB antibody
      • H3FC antibody
      • H3FD antibody
      • H3FH antibody
      • H3FI antibody
      • H3FK antibody
      • HIST1 cluster, H3A antibody
      • HIST1 cluster, H3B antibody
      • HIST1 cluster, H3C antibody
      • HIST1 cluster, H3D antibody
      • HIST1 cluster, H3F antibody
      • HIST1 cluster, H3G antibody
      • HIST1 cluster, H3H antibody
      • HIST1 cluster, H3I antibody
      • HIST1 cluster, H3J antibody
      • HIST1H3A antibody
      • HIST1H3B antibody
      • HIST1H3C antibody
      • HIST1H3D antibody
      • HIST1H3E antibody
      • HIST1H3F antibody
      • HIST1H3G antibody
      • HIST1H3H antibody
      • HIST1H3I antibody
      • HIST1H3J antibody
      • HIST3H3 antibody
      • Histone 1, H3a antibody
      • Histone 1, H3b antibody
      • Histone 1, H3c antibody
      • Histone 1, H3d antibody
      • Histone 1, H3e antibody
      • Histone 1, H3f antibody
      • Histone 1, H3g antibody
      • Histone 1, H3h antibody
      • Histone 1, H3i antibody
      • Histone 3, H3 antibody
      • histone cluster 1 H3 family member a antibody
      • histone cluster 1 H3 family member b antibody
      • histone cluster 1 H3 family member c antibody
      • histone cluster 1 H3 family member d antibody
      • histone cluster 1 H3 family member e antibody
      • histone cluster 1 H3 family member f antibody
      • histone cluster 1 H3 family member g antibody
      • histone cluster 1 H3 family member h antibody
      • histone cluster 1 H3 family member i antibody
      • histone cluster 1 H3 family member j antibody
      • Histone cluster 1, H3a antibody
      • Histone cluster 1, H3b antibody
      • Histone cluster 1, H3c antibody
      • Histone cluster 1, H3d antibody
      • Histone cluster 1, H3e antibody
      • Histone cluster 1, H3f antibody
      • Histone cluster 1, H3g antibody
      • Histone cluster 1, H3i antibody
      • Histone cluster 1, H3j antibody
      • Histone gene cluster 1, H3 histone family, member A antibody
      • Histone gene cluster 1, H3 histone family, member B antibody
      • Histone gene cluster 1, H3 histone family, member C antibody
      • Histone gene cluster 1, H3 histone family, member D antibody
      • Histone gene cluster 1, H3 histone family, member F antibody
      • Histone gene cluster 1, H3 histone family, member G antibody
      • Histone gene cluster 1, H3 histone family, member H antibody
      • Histone gene cluster 1, H3 histone family, member I antibody
      • Histone gene cluster 1, H3 histone family, member J antibody
      • Histone gene cluster 1, H3A antibody
      • Histone gene cluster 1, H3B antibody
      • Histone gene cluster 1, H3C antibody
      • Histone gene cluster 1, H3D antibody
      • Histone gene cluster 1, H3E antibody
      • Histone gene cluster 1, H3F antibody
      • Histone gene cluster 1, H3G antibody
      • Histone gene cluster 1, H3H antibody
      • Histone gene cluster 1, H3I antibody
      • Histone gene cluster 1, H3J antibody
      • Histone H 3 antibody
      • Histone H3.1 antibody
      • Histone H3.2 antibody
      • Histone H3.3 antibody
      • Histone H3/a antibody
      • Histone H3/b antibody
      • Histone H3/c antibody
      • Histone H3/d antibody
      • Histone H3/f antibody
      • Histone H3/h antibody
      • Histone H3/i antibody
      • Histone H3/j antibody
      • Histone H3/k antibody
      • Histone H3/l antibody
      • Histone H3/m antibody
      see all

    Anti-Histone H3 (acetyl K27) antibody [EP865Y] 图像

    • All lanes : Anti-Histone H3 (acetyl K27) antibody [EP865Y] (ab45173) at 1/500 dilution

      Lane 1 : C6 cell lysate, untreated
      Lane 2 : C6 cell lysate, treated with TSA

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 15 kDa
      Observed band size : 17 kDa (why is the actual band size different from the predicted?)
    • Ab45173 (1:100) staining human Histone H3T in human hepatocellular carcinoma by immunohistochemistry using paraffin embedded tissue.
    • Immunofluorescent staining of A431 cells using ab45173 (1:100).

    Anti-Histone H3 (acetyl K27) antibody [EP865Y] (ab45173)参考文献

    This product has been referenced in:
    • Azad GK  et al. Assessment of the biological pathways targeted by isocyanate using N-succinimidyl N-methylcarbamate in budding yeast Saccharomyces cerevisiae. PLoS One 9:e92993 (2014). WB ; Chicken . Read more (PubMed: 24664350) »
    • Azad GK  et al. Depletion of cellular iron by curcumin leads to alteration in histone acetylation and degradation of Sml1p in Saccharomyces cerevisiae. PLoS One 8:e59003 (2013). WB ; Saccharomyces cerevisiae . Read more (PubMed: 23520547) »

    See all 4 Publications for this product

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