Anti-Histone H2B (phospho S32)抗体(ab10476)
- 数据表
- 具体的参考文献 (3)
- 实验方案
概述
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产品名称Anti-Histone H2B (phospho S32)抗体
参阅全部 Histone H2B 一抗 -
描述兔多克隆抗体to Histone H2B (phospho S32)
该产品是 快线抗体，通过亲和纯化得到。对抗原肽有高灵敏度及特异性（ELISA测试）。 Read the terms of use »
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宿主Rabbit
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种属反应性与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H2B, phosphorylated at S32.
(Peptide available as ab18504.)
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常规说明
Serine 32 of Histone H2B was highlighted as being highly conserved in Cheung et al, indicating that it might be a good candidate phosphorylation site.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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Primary antibody说明Serine 32 of Histone H2B was highlighted as being highly conserved in Cheung et al, indicating that it might be a good candidate phosphorylation site.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/200. | |
| ELISA | Use at an assay dependent concentration. : This antibody gave a positive result in ELISA against the immunizing peptide (ab18504). |
靶标
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相关性Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in the formation of higher order structure in chromatin and the maintenance of overall chromatin compaction. Whilst the core histones are highly conserved across a wide range of organisms, the linker histones are less conserved.
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细胞定位Nuclear
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数据库链接
- Entrez Gene: 10339 Human
- Entrez Gene: 10340 Human
- Entrez Gene: 54145 Human
- Entrez Gene: 104021 Mouse
- SwissProt: P33778 Human
- SwissProt: P58876 Human
- SwissProt: Q16778 Human
- SwissProt: Q93079 Human
see all -
别名
- H2b.1A antibody
- HIST1H2BD antibody
- HIST1H2BH antibody
see all
图片
This Fast-Track antibody is not yet fully characterised. These images represent
inconclusive preliminary data.
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Western blot - Anti-Histone H2B (phospho S32) antibody (ab10476)All lanes : Anti-Histone H2B (phospho S32) antibody (ab10476) at 1/500 dilution
Lane 1 : Hela control
Lane 2 : Gamma irraditaed hela 1h
Lane 3 : Gamma irraditaed hela 2h
Lane 4 : Hela control with Human Histone H2B (phospho S32) peptide (ab18504) at 1 µg/ml
Lane 5 : Gamma irraditaed hela 1h with Human Histone H2B (phospho S32) peptide (ab18504) at 1 µg/ml
Lane 6 : Gamma irraditaed hela 2h with Human Histone H2B (phospho S32) peptide (ab18504) at 1 µg/ml
Lane 7 : Hela control with Human Histone H2B peptide (ab18507) at 1 µg/ml
Lane 8 : Gamma irraditaed hela 1h with Human Histone H2B peptide (ab18507) at 1 µg/ml
Lane 9 : Gamma irraditaed hela 2h with Human Histone H2B peptide (ab18507) at 1 µg/ml
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Performed under reducing conditions.
Observed band size: 15 kDa (why is the actual band size different from the predicted?)
Exposure time: 1 minuteWestern blot using ab10476 at 1/500 on HeLa lysates taken at various time points after gamma irradiation (ab13823).
Lane 1 : Hela control
Lane 2 : Gamma irraditaed hela 1h
Lane 3 : Gamma irraditaed hela 2h
Lane 4 : Hela control + Histone H2B peptide - phospho S32 (ab18504) -
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (phospho S32) antibody (ab10476)ICC/IF image of ab10476 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab10476, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2, Hek293 and MCF7 cells fixed in 4% PFA at 1ug/ml and Hela, Hek293, HepG2 and MCF7 cells fixed in 100% methanol at 1ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B (phospho S32) antibody (ab10476)IHC image of ab10476 staining in Human Breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10476, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (phospho S32) antibody (ab10476)Image courtesy of an abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canadaab10476 (1/200) staining Histone H2B phospho S32 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). for further experimental details please refer to Abreview.
文献
This product has been referenced in:
- Li J et al. Nuclear PKC-? facilitates rapid transcriptional responses in human memory CD4+ T cells through p65 and H2B phosphorylation. J Cell Sci 129:2448-61 (2016). WB, ICC/IF ; Human . Read more (PubMed: 27149922) »
- Lau AT et al. Phosphorylation of histone H2B serine 32 is linked to cell transformation. J Biol Chem : (2011). WB, ICC/IF ; Human . Read more (PubMed: 21646345) »
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