Anti-Histone H2B抗体[EP2327] (ab76246)


  • 产品名称Anti-Histone H2B抗体[EP2327]
    参阅全部 Histone H2B 一抗
  • 描述
    兔单克隆抗体[EP2327] to Histone H2B
  • 特异性Please note that this sequence is not unique to Histone H2B and that this antibody will recognise other isoforms.
  • 经测试应用适用于: WB, IHC-P, ICCmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Histone H2B (N terminal).
    Database link: P33778

  • 阳性对照
    • WB: Jurkat nuclear lysate IHC-P: Human transitional cell carcinoma of urinary bladder tissue
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EP2327
  • 同种型IgG
  • 研究领域



Our Abpromise guarantee covers the use of ab76246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 14 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Use of HRP conjugated or polymerized HRP secondary antibodies is recommended. Stronger signals have been found using the polymerized HRP secondary.

ICC 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 相关性Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
    • 细胞定位Nuclear
    • 数据库链接
    • 别名
      • H2B GL105 antibody
      • H2B histone family member O antibody
      • H2B histone family member S antibody
      • H2B.1 antibody
      • H2B.1 B antibody
      • H2B.b antibody
      • H2B.c antibody
      • H2B.d antibody
      • H2B.e antibody
      • H2B.f antibody
      • H2B.j antibody
      • H2B.q antibody
      • H2B/b antibody
      • H2B/c antibody
      • H2B/d antibody
      • H2B/e antibody
      • H2B/f antibody
      • H2B/j antibody
      • H2B/o antibody
      • H2B/q antibody
      • H2BFB antibody
      • H2BFC antibody
      • H2BFD antibody
      • H2BFE antibody
      • H2BFF antibody
      • H2BFJ antibody
      • H2BFO antibody
      • H2BFQ antibody
      • H2BFS antibody
      • HIRIP2 antibody
      • HIST1H2BB antibody
      • HIST1H2BD antibody
      • HIST1H2BH antibody
      • HIST1H2BL antibody
      • HIST1H2BM antibody
      • HIST1H2BN antibody
      • HIST2H2BE antibody
      • Histone H2B antibody
      • Histone H2B type 1 B antibody
      • Histone H2B type 1 D antibody
      • Histone H2B type 1 H antibody
      • Histone H2B type 1 L antibody
      • Histone H2B type 1 M antibody
      • Histone H2B type 1 N antibody
      • Histone H2B type 2 E antibody
      • histone protein antibody
      see all

    Anti-Histone H2B antibody [EP2327] 图像

    • Anti-Histone H2B antibody [EP2327] (ab76246) at 1/1000 dilution + Jurkat cell lysate at 10 µg

      goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size : 14 kDa
      Observed band size : 14 kDa
    • ab76246, at a 1/100 dilution, staining human Histone H2B type 1B in transitional cell carcinoma of urinary bladder, by Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

    Anti-Histone H2B antibody [EP2327] (ab76246)参考文献

    ab76246 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76246.
    Please use the links above to contact us or submit feedback about this product.