RabMAb

Anti-Histone H2B (acetyl K5)抗体[EP857Y] - ChIP Grade (ab40886)

概述

  • 产品名称Anti-Histone H2B (acetyl K5)抗体[EP857Y] - ChIP Grade
    参阅全部 Histone H2B 一抗
  • 描述
    兔单克隆抗体[EP857Y] to Histone H2B (acetyl K5) - ChIP Grade
  • 特异性There is cross-reactivity with H3K27Ac (Histone H3 acetylated on Lys 27).
  • 经测试应用适用于: WB, IHC - Wholemount, CHIPseq, ChIP, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human, Caenorhabditis elegans
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Histone H2B aa 1-100 (acetyl K5).

  • 阳性对照
    • TSA-treated A431 cell lysate, human urinary bladder carcinoma.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab40886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
IHC - Wholemount Use at an assay dependent concentration.
CHIPseq Use at an assay dependent concentration.
ChIP Use 6-25 µg for µg of chromatin.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/250 - 1/500.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade 图像

    • Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 6µl of ab40886 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.    

    • All lanes : Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886) at 1/50000 dilution

      Lane 1 : A431 cell lysate - untreated
      Lane 2 : A431 cell lysate - treated with TSA

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 14 kDa
      Observed band size : 14 kDa
    • ab40886 staining human bladder carcinoma for Histone H2B expression (1:250 dilution)
    • ab40886 (1:250) staining A431 cells by immunofluorescence.
    • ab40886 at a 1/600 dilution for ChIP analysis of mouse dorsal skin epidermis whole tissue lysate, incubated for 15 hours at 4°C with ChIP dilution buffer. Cross-linking (X-ChIP) using 1% formaldehyde for 10 minutes.
      Detection step: Semiquantitative PCR.
      Negative control: Rabbit IgG.
      Cells treated with active vitamin D3.

      See Abreview

    • IHC - Wholemount of Caenorhabditis elegans larvae labelling Histone H2B (acetyl K5) with ab40886. The sample was incubated with primary antibody (1/200 in PBS + 3% BSA + 0.1% Triton X-100) for 12 hours at 4°C. ab150077, a goat anti-rabbit Alexa 488 (1/1000), was used as the secondary antibody.

      See Abreview

    Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade (ab40886)参考文献

    This product has been referenced in:
    • Wang Z  et al. Combinatorial patterns of histone acetylations and methylations in the human genome. Nat Genet 40:897-903 (2008). CHIPseq . Read more (PubMed: 18552846) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (12.5%)
    Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Fruit fly larvae)
    Specification Fruit fly larvae
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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    提交于 Jan 08 2015

    Application IHC - Wholemount
    Sample Caenorhabditis elegans Embryo (C. elegans larvae)
    Specification C. elegans larvae
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    提交于 Dec 30 2013

    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
    Antigen retrieval step None
    Sample Rat Tissue sections (Rat olfactory bulb frozen section)
    Specification Rat olfactory bulb frozen section
    Permeabilization Yes - 0.1% Triton X-100
    Fixative Paraformaldehyde
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    提交于 Dec 30 2013

    Application Immunocytochemistry
    Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 23°C
    Sample Human Cultured Cells (Human embryonic stem cells)
    Specification Human embryonic stem cells
    Permeabilization Yes - 0.3% Triton X-100
    Fixative Paraformaldehyde
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    提交于 Dec 27 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application ChIP
    Sample Mouse Tissue lysate - whole (dorsal skin epidermis)
    Specification dorsal skin epidermis
    Type Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% formaldehyde
    Detection step Semiquantitative PCR
    Negative control Rabbit IgG
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    提交于 Feb 15 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"