Anti-Histone H2B (acetyl K5)抗体(ab61227)

概述

  • 产品名称Anti-Histone H2B (acetyl K5)抗体
    参阅全部 Histone H2B 一抗
  • 描述
    兔多克隆抗体to Histone H2B (acetyl K5)
  • 特异性ab61227 detects Histone H2B only when acetylated at lysine 5.
  • 经测试应用适用于: ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide within Human Histone H2B (acetyl K5). The exact sequence is proprietary.
    Sequence:

    PAK(Ac)SA


    Database link: P57053

  • 阳性对照
    • Human breast carcinoma tissue. HeLa cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab61227 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab61227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

靶标

Anti-Histone H2B (acetyl K5) antibody 图像

  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Histone H2B (acetyl K5) antibody (ab61227) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of immunising acetylated peptide.
  • Immunofluorescence analysis of HeLa cells, using Histone H2B (acetyl K5) antibody (ab61227) at 1/500 - 1/1000 dilution, in the presence (right panel) and absence (left panel) of immunising acetylated peptide.

Anti-Histone H2B (acetyl K5) antibody (ab61227)参考文献

ab61227 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"