Anti-Histone H2B (acetyl K16)抗体- ChIP Grade (ab40977)

概述

  • 产品名称Anti-Histone H2B (acetyl K16)抗体- ChIP Grade
    参阅全部 Histone H2B 一抗
  • 描述
    兔多克隆抗体to Histone H2B (acetyl K16) - ChIP Grade
  • 经测试应用适用于: IHC-P, WB, ChIP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Chicken, Cow, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H2B, acetylated at K16.

    (Peptide available as ab41879.)

  • 阳性对照
    • ab40977 gave a positive signal in the following lysate: HeLa Histone Preparation Nuclear Lysate - Butyrated treated

性能

应用

Our Abpromise guarantee covers the use of ab40977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 14 kDa).
ChIP Use a concentration of 4 µg/ml. PubMed: 20693536
ICC/IF Use a concentration of 1 µg/ml.

靶标

Anti-Histone H2B (acetyl K16) antibody - ChIP Grade 图像

  • All lanes : Anti-Histone H2B (acetyl K16) antibody - ChIP Grade (ab40977) at 1 µg/ml

    Lane 1 : HeLa Histone Preparation Nuclear Lysate
    Lane 2 : HeLa Histone Preparation Nuclear Lysate - Butyrated treated
    Lane 3 : HeLa Histone Preparation Nuclear Lysate with Human Histone H2B (acetyl K16) peptide (ab41879) at 1 µg/ml
    Lane 4 : HeLa Histone Preparation Nuclear Lysate - Butyrated treated with Human Histone H2B (acetyl K16) peptide (ab41879) at 1 µg/ml
    Lane 5 : HeLa Histone Preparation Nuclear Lysate with Human Histone H2B peptide (ab35793) at 1 µg/ml
    Lane 6 : HeLa Histone Preparation Nuclear Lysate - Butyrated treated with Human Histone H2B peptide (ab35793) at 1 µg/ml

    Lysates/proteins at 2.5 µg per lane.

    Secondary
    HRP Conjugated Goat Anti-Rabbit IgG at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 14 kDa
    Observed band size : 16 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab40977 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab40977, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • IHC image of ab40977 staining in cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40977, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histone H2B (acetyl K16) antibody - ChIP Grade (ab40977)参考文献

This product has been referenced in:
  • Singh P  et al. Coordinated allele-specific histone acetylation at the differentially methylated regions of imprinted genes. Nucleic Acids Res 38:7974-90 (2010). ChIP ; Mouse . Read more (PubMed: 20693536) »

See 1 Publication for this product

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