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RabMAb

Anti-Histone H2B (acetyl K15)抗体[EP955Y] (ab62335)

概述

  • 产品名称
    Anti-Histone H2B (acetyl K15)抗体[EP955Y]
    参阅全部 Histone H2B 一抗
  • 描述
    兔单克隆抗体[EP955Y] to Histone H2B (acetyl K15)
  • 特异性
    This antibody only detects Histone H2B acetylated on Lysine 15.
  • 经测试应用
    适用于: Flow Cyt, ICC/IF, WB, IHC-Pmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Histone H2B aa 1-100 (acetyl K15).

  • 阳性对照
    • HeLa cell lysate or human breast carcinoma tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度
    Tissue culture supernatant
  • 克隆
    单克隆
  • 克隆编号
    EP955Y
  • 同种型
    IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab62335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/500.
WB 1/2000 - 1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).
IHC-P 1/100 - 1/250.
  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 功能
      Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    • 序列相似性
      Belongs to the histone H2B family.
    • 翻译后修饰
      Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.
      Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.
    • 细胞定位
      Nucleus. Chromosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • H2B 1A antibody
      • H2B antibody
      • H2B histone family antibody
      • H2B2f antibody
      • H2B2F_HUMAN antibody
      • H2Ba antibody
      • H2Bf antibody
      • HIST2H2BF antibody
      • histone H2B antibody
      • histone H2B type 1 antibody
      • Histone H2B type 2-F antibody
      • MGC131639 antibody
      see all

    图片

    • All lanes : Anti-Histone H2B (acetyl K15) antibody [EP955Y] (ab62335) at 1/2000 dilution

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate (treated with TSA)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP conjugate at 1/2000 dilution

      Predicted band size : 14 kDa
      Observed band size : 17 kDa (why is the actual band size different from the predicted?)
    • Ab62335 (1/100-1/250) staining human Histone H2B in human breast carcinoma tissue by immunohistochemistry using paraffin embededd tissue.
    • ab62335 (1/500) staining Histone H2B (acetyl K15) in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.

      See Abreview

    文献

    This product has been referenced in:

    See 1 Publication for this product

    客户评价及客户问答

    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein G
    Sample
    Human Cell lysate - whole cell (HEK293T cells)
    Specification
    HEK293T cells
    Total protein in input
    5 µg
    Username

    Abcam user community

    Verified customer

    提交于 Dec 20 2013

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Antigen retrieval step
    None
    Sample
    Mouse Tissue sections (Mouse thymus tissue)
    Specification
    Mouse thymus tissue
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Dec 20 2013

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (15%)
    Sample
    Rat Cell lysate - other (Chromatin extracted from thymocytes)
    Specification
    Chromatin extracted from thymocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 Dec 20 2013

    Application
    Immunocytochemistry
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample
    Mouse Cultured Cells (Mouse embryonic neural stem cells)
    Specification
    Mouse embryonic neural stem cells
    Permeabilization
    Yes - 0.3% Triton X-100
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Dec 20 2013

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (12.5%)
    Sample
    Mouse Cell lysate - whole cell (Mouse embryonic neural stem cells)
    Specification
    Mouse embryonic neural stem cells
    Treatment
    Withdraw 10ug/ml FGF2
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 Dec 20 2013



    1.) I can confirm that this was not tested experimentally. The exact immunogenic sequence of ab139383 is 15 amino acids long. Alignment of the immunogenic sequence with the sequence for P70696 (mouse TH2B) is as follows:

    80.0% iden...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton-X100 in PBS
    Username

    Dr. Kirk Mcmanus

    Verified customer

    提交于 Nov 11 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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