RabMAb

Anti-Histone H2B (acetyl K15)抗体[EP955Y] (ab62335)

概述

  • 产品名称Anti-Histone H2B (acetyl K15)抗体[EP955Y]
    参阅全部 Histone H2B 一抗
  • 描述
    兔单克隆抗体[EP955Y] to Histone H2B (acetyl K15)
  • 特异性This antibody only detects Histone H2B acetylated on Lysine 15.
  • 经测试应用适用于: Flow Cyt, ICC/IF, WB, IHC-Pmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Histone H2B aa 1-100 (acetyl K15).

  • 阳性对照
    • HeLa cell lysate or human breast carcinoma tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EP955Y
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab62335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/500.
WB 1/2000 - 1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).
IHC-P 1/100 - 1/250.
  • 应用说明Is unsuitable for IP.
  • 靶标

    Anti-Histone H2B (acetyl K15) antibody [EP955Y] 图像

    • All lanes : Anti-Histone H2B (acetyl K15) antibody [EP955Y] (ab62335) at 1/2000 dilution

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate (treated with TSA)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP conjugate at 1/2000 dilution

      Predicted band size : 14 kDa
      Observed band size : 17 kDa (why is the actual band size different from the predicted?)
    • Ab62335 (1/100-1/250) staining human Histone H2B in human breast carcinoma tissue by immunohistochemistry using paraffin embededd tissue.
    • ab62335 (1/500) staining Histone H2B (acetyl K15) in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.

      See Abreview

    Anti-Histone H2B (acetyl K15) antibody [EP955Y] (ab62335)参考文献

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Application Immunoprecipitation
    Immuno-precipitation step Protein G
    Sample Human Cell lysate - whole cell (HEK293T cells)
    Specification HEK293T cells
    Total protein in input 5 µg
    Username

    Abcam user community

    Verified customer

    提交于 Dec 20 2013

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Antigen retrieval step None
    Sample Mouse Tissue sections (Mouse thymus tissue)
    Specification Mouse thymus tissue
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Dec 20 2013

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (15%)
    Sample Rat Cell lysate - other (Chromatin extracted from thymocytes)
    Specification Chromatin extracted from thymocytes
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 Dec 20 2013

    Application Immunocytochemistry
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample Mouse Cultured Cells (Mouse embryonic neural stem cells)
    Specification Mouse embryonic neural stem cells
    Permeabilization Yes - 0.3% Triton X-100
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Dec 20 2013

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (12.5%)
    Sample Mouse Cell lysate - whole cell (Mouse embryonic neural stem cells)
    Specification Mouse embryonic neural stem cells
    Treatment Withdraw 10ug/ml FGF2
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 Dec 20 2013



    1.) I can confirm that this was not tested experimentally. The exact immunogenic sequence of ab139383 is 15 amino acids long. Alignment of the immunogenic sequence with the sequence for P70696 (mouse TH2B) is as follows:

    80.0% iden...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton-X100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    提交于 Nov 11 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"