RabMAb

Anti-Histone H2B (acetyl K12)抗体[EP858Y] (ab40883)

概述

  • 产品名称Anti-Histone H2B (acetyl K12)抗体[EP858Y]
    参阅全部 Histone H2B 一抗
  • 描述
    兔单克隆抗体[EP858Y] to Histone H2B (acetyl K12)
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Histone H2B aa 1-100 (acetyl K12).

  • 阳性对照
    • HeLa cells, Human colon carcinoma. IHC-P: Human normal colon FFPE tissue sections.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    This antibody only detects Histone H2B acetylated on Lysine 12.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • Primary antibody说明This antibody only detects Histone H2B acetylated on Lysine 12.
  • 克隆单克隆
  • 克隆编号EP858Y
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab40883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 15 kDa (predicted molecular weight: 14 kDa).
ICC/IF 1/250 - 1/500.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    • 序列相似性Belongs to the histone H2B family.
    • 翻译后修饰Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.
      Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.
    • 细胞定位Nucleus. Chromosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • H2B 1A antibody
      • H2B antibody
      • H2B histone family antibody
      • H2B2f antibody
      • H2B2F_HUMAN antibody
      • H2Ba antibody
      • H2Bf antibody
      • HIST2H2BF antibody
      • histone H2B antibody
      • histone H2B type 1 antibody
      • Histone H2B type 2-F antibody
      • MGC131639 antibody
      see all

    Anti-Histone H2B (acetyl K12) antibody [EP858Y] 图像

    • IHC image of ab40883 staining Histone H2B (acetyl K12) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab40883, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883) at 1/2000 dilution + C6+TSA cell lysate at 10 µg

      Predicted band size : 14 kDa
      Observed band size : 15 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using anti-Histone H2B ab40883 at a 1:250 dilution.Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using anti-Histone H2B ab40883 at a 1:250 dilution.
    • ab40883 (1:250) staining of HeLa cells by immunofluorescence.
    • ab40883 (1/500) staining Histone H2B (acetyl K12) in assynchronous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised using 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

      See Abreview

    Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)参考文献

    ab40883 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton X100
    Username

    Dr. Kirk McManus

    Verified customer

    提交于 Sep 15 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"